Proteomic analyses reveal plasma EFEMP1 and CXCL12 as biomarkers and determinants of neurodegeneration

Alzheimers Dement. 2024 Sep;20(9):6486-6505. doi: 10.1002/alz.14142. Epub 2024 Aug 11.

Abstract

Introduction: Plasma proteomic analyses of unique brain atrophy patterns may illuminate peripheral drivers of neurodegeneration and identify novel biomarkers for predicting clinically relevant outcomes.

Methods: We identified proteomic signatures associated with machine learning-derived aging- and Alzheimer's disease (AD) -related brain atrophy patterns in the Baltimore Longitudinal Study of Aging (n = 815). Using data from five cohorts, we examined whether candidate proteins were associated with AD endophenotypes and long-term dementia risk.

Results: Plasma proteins associated with distinct patterns of age- and AD-related atrophy were also associated with plasma/cerebrospinal fluid (CSF) AD biomarkers, cognition, AD risk, as well as mid-life (20-year) and late-life (8-year) dementia risk. EFEMP1 and CXCL12 showed the most consistent associations across cohorts and were mechanistically implicated as determinants of brain structure using genetic methods, including Mendelian randomization.

Discussion: Our findings reveal plasma proteomic signatures of unique aging- and AD-related brain atrophy patterns and implicate EFEMP1 and CXCL12 as important molecular drivers of neurodegeneration.

Highlights: Plasma proteomic signatures are associated with unique patterns of brain atrophy. Brain atrophy-related proteins predict clinically relevant outcomes across cohorts. Genetic variation underlying plasma EFEMP1 and CXCL12 influences brain structure. EFEMP1 and CXCL12 may be important molecular drivers of neurodegeneration.

Keywords: Alzheimer's disease; biomarkers; inflammation; neurodegeneration; proteomics.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Aging
  • Alzheimer Disease* / blood
  • Alzheimer Disease* / pathology
  • Atrophy / pathology
  • Biomarkers* / blood
  • Brain* / pathology
  • Chemokine CXCL12* / blood
  • Cohort Studies
  • Extracellular Matrix Proteins* / blood
  • Female
  • Humans
  • Longitudinal Studies
  • Male
  • Middle Aged
  • Proteomics*

Substances

  • Biomarkers
  • Chemokine CXCL12
  • EFEMP1 protein, human
  • Extracellular Matrix Proteins
  • CXCL12 protein, human