Transcriptomics in the Study of Antiviral Innate Immunity

Qingcong Zheng; Rongjie Lin; Chunfu Zheng

doi:10.1007/978-1-0716-4108-8_10

Transcriptomics in the Study of Antiviral Innate Immunity

Methods Mol Biol. 2025:2854:83-91. doi: 10.1007/978-1-0716-4108-8_10.

Authors

Qingcong Zheng^#¹, Rongjie Lin^#², Chunfu Zheng³

Affiliations

¹ Department of Spinal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
² Department of Orthopedic Surgery, Fujian Medical University Union Hospital, Fuzhou, China.
³ Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.

^# Contributed equally.

PMID: 39192121
DOI: 10.1007/978-1-0716-4108-8_10

Abstract

Transcriptomics is an extremely important area of molecular biology and is a powerful tool for studying all RNA molecules in an organism. Conventional transcriptomic technologies include microarrays and RNA sequencing, and the rapid development of single-cell sequencing and spatial transcriptomics in recent years has provided an enormous scope for research in this field. This chapter describes the application, significance, and experimental procedures of a variety of transcriptomic technologies in antiviral natural immunity.

Keywords: Antiviral innate immunity; Transcriptomics; Virus.

MeSH terms

Animals
Gene Expression Profiling* / methods
Humans
Immunity, Innate* / genetics
Oligonucleotide Array Sequence Analysis / methods
Sequence Analysis, RNA / methods
Single-Cell Analysis / methods
Transcriptome*
Virus Diseases / genetics
Virus Diseases / immunology