Two "turn on" fluorescence probes based on nitroso recognition group for detecting hydrogen sulfide

Abstract

Hydrogen sulfide is a vital signaling molecule which holds a pivotal position in numerous biological functions. In this research, two novel "OFF-ON" fluorescence probes named YNO and TNO were designed based on the nitroso recognition group to detect H₂S. Both YNO and TNO performed outstanding response rate and linear relationship between the fluorescence intensity and the concentration of H₂S. YNO possessed larger Stokes shift and longer emission wavelength. TNO had lower limit of detection. In addition, YNO was successful applied to sense endogenous and exogenous H₂S and target endoplasmic reticulum (ER) in Hela cells.

Keywords: Cell imaging; Fluorescence probe; Hydrogen sulfide; Nitroso.