Protocol to study electrophysiological properties of hPSC-derived 3D cardiac organoids using MEA and sharp electrode techniques

STAR Protoc. 2024 Dec 20;5(4):103406. doi: 10.1016/j.xpro.2024.103406. Epub 2024 Nov 7.

Abstract

Continuing advancements in human pluripotent stem cell (hPSC)-derived complex three-dimensional (3D) cardiac tissues require the development of novel technologies or adaptation of existing technologies to understand the physiology of the derived 3D cardiac tissues. In this protocol, we describe the use of multielectrode array (MEA) and sharp electrode electrophysiology techniques to investigate the electrical properties of 3D cardiac organoids. This protocol deciphers the electrical behavior of 3D cardiac organoids at both the single-cell level and tissue level.

Keywords: Cell Biology; Cell culture; Organoids; Stem Cells; Tissue Engineering.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Culture Techniques / methods
  • Electrodes
  • Electrophysiological Phenomena*
  • Heart / physiology
  • Humans
  • Microelectrodes
  • Myocardium / cytology
  • Myocytes, Cardiac / cytology
  • Myocytes, Cardiac / physiology
  • Organoids* / cytology
  • Organoids* / physiology
  • Pluripotent Stem Cells* / cytology