Transcriptional Profiling of Filovirus-Infected Nonhuman Primate Samples

Joydeep Nag; Emily Speranza

doi:10.1007/978-1-0716-4256-6_26

Transcriptional Profiling of Filovirus-Infected Nonhuman Primate Samples

Methods Mol Biol. 2025:2877:369-385. doi: 10.1007/978-1-0716-4256-6_26.

Authors

Joydeep Nag¹, Emily Speranza²

Affiliations

¹ Florida Research and Innovation Center, Cleveland Clinic, Port Saint Lucie, FL, USA.
² Florida Research and Innovation Center, Cleveland Clinic, Port Saint Lucie, FL, USA. sperane@ccf.org.

PMID: 39585635
DOI: 10.1007/978-1-0716-4256-6_26

Abstract

Transcriptional analysis is a powerful tool to help decipher the host response to filovirus infections. In this chapter, we describe how transcriptional analysis can be performed on samples obtained from nonhuman primates infected with filoviruses, specifically Marburg virus, for assessment of the RNA changes over the course of the infection and disease.

Keywords: Host response; Marburg virus; NHP; RNA-seq; Transcriptomics.

MeSH terms

Animals
Filoviridae / genetics
Filoviridae Infections / genetics
Filoviridae Infections / virology
Gene Expression Profiling / methods
Marburg Virus Disease / genetics
Marburg Virus Disease / virology
Marburgvirus* / genetics
Primates* / genetics
Primates* / virology
RNA, Viral / genetics
Transcriptome

Substances

RNA, Viral