Protocol for in vitro observation of HDAC4 condensation during induced cardiac reprogramming

Abstract

Here, we present a protocol to monitor and stimulate cardiac reprogramming via the disruption of Hdac4 condensates mediated by PC14-3-3 activation. We describe steps for inducing cardiac reprogramming in vitro, validating condensate formation ability of Hdac4, and observing Hdac4 condensates during reprogramming. We then detail procedures for disrupting these condensates using PC14-3-3 activation strategies. For complete details on the use and execution of this protocol, please refer to Liu et al.¹.

Keywords: cell culture; cell differentiation; cell isolation; classification description: cell biology; molecular biology.