Anti-hyperuricemia effects of a polysaccharide-protein complex from Lentinula edodes mediated by gut-kidney axis

Xinyi Xiong; Peng Liu; Liping Liu; Jie Feng; Jingsong Zhang; Mengqiu Yan; Yanfang Liu

doi:10.1016/j.ijbiomac.2024.139370

Anti-hyperuricemia effects of a polysaccharide-protein complex from Lentinula edodes mediated by gut-kidney axis

Int J Biol Macromol. 2025 Mar:293:139370. doi: 10.1016/j.ijbiomac.2024.139370. Epub 2024 Dec 30.

Authors

Xinyi Xiong¹, Peng Liu², Liping Liu³, Jie Feng³, Jingsong Zhang⁴, Mengqiu Yan⁵, Yanfang Liu⁶

Affiliations

¹ Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; National Engineering Research Center of Edible Fungi; National R&D Center for Edible Fungi Processing, Shanghai 201403, China.
² Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, China; National Engineering Research Center of Edible Fungi; National R&D Center for Edible Fungi Processing, Shanghai 201403, China. Electronic address: liupeng@saas.sh.cn.
³ Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, China; National Engineering Research Center of Edible Fungi; National R&D Center for Edible Fungi Processing, Shanghai 201403, China.
⁴ Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, China; National Engineering Research Center of Edible Fungi; National R&D Center for Edible Fungi Processing, Shanghai 201403, China. Electronic address: syja16@saas.sh.cn.
⁵ Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, China; National Engineering Research Center of Edible Fungi; National R&D Center for Edible Fungi Processing, Shanghai 201403, China. Electronic address: yanmengqiu@saas.sh.cn.
⁶ Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, China; National Engineering Research Center of Edible Fungi; National R&D Center for Edible Fungi Processing, Shanghai 201403, China. Electronic address: aliu-1980@163.com.

PMID: 39743059
DOI: 10.1016/j.ijbiomac.2024.139370

Abstract

In order to investigate the effects of Lentinula edodes (L. edodes) on hyperuricemia, a polysaccharide-protein complex (LEPP) was prepared and characterized, and the activity was also systematically studied. LEPP was mainly composed of proteins (18.55 %) and polysaccharides (36.98 %) with three polysaccharide fractions (molecular weights were 8.98 × 10⁶, 7.34 × 10⁷ and 1.15 × 10⁷ g/mol). The experiment results showed that 250-1000 mg/kg/day LEPP could decrease the serum uric acid (UA) level from 281.62 to 109.42-138.12 μmol/L (p < 0.001), and there were significant differences between high and low doses (p < 0.05). LEPP simultaneously improved renal function, relieved oxidative stress and inflammation in the kidney and regulated renal UA transporters (URAT1, GLUT9, OAT1 and ABCG2) of hyperuricemia rats. Furthermore, gut microbiota dysbiosis and intestinal barrier function disruption were modified by LEPP. Among them, LEPP could reverse the relative abundances of Parabacteroides, Lachnospiraceae, Colidextribacter, UCG-010, Monoglobus, Lactobacillus and Muribaculaceae, which were associated with regulating the UA level, renal function and the expression of renal UA transporters in hyperuricemia rats through correlation analysis. Conclusively, LEPP prevented the pathological process of hyperuricemia through regulating the gut-kidney axis, which provided its complementary role in existing anti-hyperuricemia treatment strategies.

Keywords: Gut microbiota; Gut-kidney axis; Hyperuricemia; Lentinula edodes; Polysaccharide-protein complex.

MeSH terms

Animals
Gastrointestinal Microbiome / drug effects
Hyperuricemia* / blood
Hyperuricemia* / drug therapy
Hyperuricemia* / metabolism
Kidney* / drug effects
Kidney* / metabolism
Male
Oxidative Stress / drug effects
Polysaccharides* / chemistry
Polysaccharides* / pharmacology
Rats
Shiitake Mushrooms* / chemistry
Uric Acid / blood

Substances

Uric Acid
Polysaccharides