In recent years, quinoa protein (QP) has attracted attention for its balanced amino acids composition, but its limited techno-functional properties continue to pose challenges for its utilization. Non-enzymatic Maillard glycation is considered as a promising strategy to expand the utilization of plant proteins in food processing due to its cost-effectiveness, spontaneous nature, and the lack of need for additives to initiate the reaction. Furthermore, the use of hyaluronic acid (HA) as an ingredient in food products is becoming increasingly accepted and popular. Therefore, the present study aims to prepare QP-HA glyconjugates by wet heating and to investigate the effects of sugar/protein ratios and reaction times on the structural features and functional properties of QP. The results showed that heating time and sugar/protein concentration ratio obviously affected the degree of grafting, structure and hydrophobicity of the conjugates. The random coil content of QP-HA increased significantly, resulting in a more flexible structure after Maillard glycation. After 3 h of glycation reaction, the QP-HA conjugates showed better emulsification, solubility, thermal stability and antioxidant activity compared to QP. Accordingly, these results indicate that polysaccharide-induced Maillard reaction is a potentially attractive approach for selective functionality enhancement and nutraceutical development of QP, which provides a new way to expand the application range of QP.
Keywords: Functional properties; Maillard glycation; Protein structure; Quinoa protein-hyaluronic acid conjugates; Thermal stability.
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