CD40-TRAF6 inhibition suppresses cardiovascular inflammation, oxidative stress and functional complications in a mouse model of arterial hypertension

Redox Biol. 2025 Mar:80:103520. doi: 10.1016/j.redox.2025.103520. Epub 2025 Jan 29.

Abstract

Cardiovascular disease is the leading cause of disease burden and death worldwide and is fueled by vascular inflammation. CD40L-CD40-TRAF signaling is involved in the progression of atherosclerosis and drives the development of coronary heart disease (CHD). The present study investigates whether the CD40L-CD40-TRAF6 signaling pathway with focus on immune cells and adipocytes could be a therapeutic target in arterial hypertension. Arterial hypertension was induced in WT (C57BL6/J) and cell-specific CD40(L) knockout mice (AdipoqCre x CD40 fl/fl, CD4Cre x CD40 fl/fl, CD19Cre x CD40 fl/fl, and GP1baCre x CD40L fl/fl) via angiotensin (AT-II) infusion (1 mg/kg/d) for seven days. Hypertensive WT mice were also treated with a CD40-TRAF6 inhibitor (2.5 mg/kg/d, for 7d). The TRAF6 inhibitor treatment normalized endothelial dysfunction and reduced blood pressure in hypertensive wild type animals. Reactive oxygen species production was decreased by TRAF6 inhibition in blood, aorta, heart, kidney, and perivascular fat tissue. Additionally, FACS analysis revealed that TRAF6 inhibition prevents immune cell migration into the aortic vessel wall observed by reduced CD45+ leukocyte, Ly6G+/Ly6C+ neutrophil, and Ly6Chigh inflammatory monocyte content. The hypertensive cell type-specific CD40(L) knockout animals showed only a minor effect on endothelial function, blood pressure, and oxidative stress. Therefore, we conclude that targeting CD40 directly on adipocytes, B-cells, T-cells, or CD40L on platelets is not a promising target to prevent hypertension complications. In summary, TRAF6 inhibition but not adipocyte, B-cell, or T-cell-specific CD40 or platelet-specific CD40L deficiency reduces pathophysiological vascular inflammation in hypertensive mice, suggesting TRAF6 inhibition as a potential therapeutic target in hypertensive patients.

Keywords: Arterial hypertension; Blood pressure; CD40(L)-TRAF6 signaling; Endothelial function; Inflammation.

MeSH terms

  • Animals
  • CD40 Antigens* / antagonists & inhibitors
  • CD40 Antigens* / genetics
  • CD40 Antigens* / metabolism
  • Disease Models, Animal
  • Hypertension* / drug therapy
  • Hypertension* / etiology
  • Hypertension* / genetics
  • Hypertension* / metabolism
  • Hypertension* / pathology
  • Inflammation* / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Oxidative Stress* / drug effects
  • Reactive Oxygen Species / metabolism
  • Signal Transduction / drug effects
  • TNF Receptor-Associated Factor 6* / antagonists & inhibitors
  • TNF Receptor-Associated Factor 6* / genetics
  • TNF Receptor-Associated Factor 6* / metabolism

Substances

  • TNF Receptor-Associated Factor 6
  • CD40 Antigens
  • TRAF6 protein, mouse
  • Reactive Oxygen Species