Proliferative retinopathy in a patient with mutation in the CLCN2 gene

Eur J Ophthalmol. 2025 May;35(3):NP46-NP52. doi: 10.1177/11206721251316612. Epub 2025 Feb 21.

Abstract

PurposeCLCN2 is a gene that encodes the voltage-gated chloride channel protein 2 in the human brain and eyes. While mutations in this gene have been associated with leukoencephalopathy as well as ocular manifestations including optic neuropathy and choroidopathy, here we report for the first time a case of severe proliferative retinopathy in a patient with CLCN2 mutation.ObservationsA 12-year-old girl with Familial Mediterranean Fever (FMF) was referred due to blurred vision in both eyes. Ophthalmoscopic examination revealed mild vitreous hemorrhage, large neovascularization at the disc (NVD), extensive neovascularization along the arcades (i.e., NVE; neovascularization elsewhere), retinal arteriolar narrowing, silver sheathing of the veins and preretinal hemorrhages. Fluorescein angiography (FA) demonstrated prominent leakage from multiple large NVEs. A thorough and multidisciplinary evaluation ruled out metabolic, thrombotic, infectious, inflammatory, or autoimmune etiologies. Notably, however, brain magnetic resonance imaging (MRI) revealed leukodystrophy yet poorly correlated with her clinical manifestation. Strikingly, whole exome sequencing uncovered a homozygous mutation in the CLCN2 gene. She was treated with intravitreal injections of the anti-vascular endothelial growth factor (VEGF) antibody Bevacizumab and laser pan-retinal photocoagulation (PRP) with partial response.ConclusionsThis case suggests that CLCN2 mutation may possess a key role in an aggressive form of proliferative retinopathy with partial response to anti-VEGF therapy. We hypothesize that the potential underlying mechanism involves astrocyte dysfunction and retinal blood barrier disruption. Noteworthy, a comprehensive approach is unequivocally significant in evaluating such cases of proliferative retinopathy with unclear etiology to establish a diagnosis and management strategy.

Keywords: CLCN2; Proliferative retinopathy; leukodystrophy; mutation.

Publication types

  • Case Reports

MeSH terms

  • Angiogenesis Inhibitors / therapeutic use
  • Bevacizumab / therapeutic use
  • CLC-2 Chloride Channels
  • Child
  • Chloride Channels* / genetics
  • Chloride Channels* / metabolism
  • DNA Mutational Analysis
  • Female
  • Fluorescein Angiography
  • Humans
  • Intravitreal Injections
  • Laser Coagulation
  • Magnetic Resonance Imaging
  • Mutation*
  • Tomography, Optical Coherence
  • Vascular Endothelial Growth Factor A / antagonists & inhibitors
  • Visual Acuity
  • Vitreoretinopathy, Proliferative* / diagnosis
  • Vitreoretinopathy, Proliferative* / genetics
  • Vitreoretinopathy, Proliferative* / therapy

Substances

  • Chloride Channels
  • CLC-2 Chloride Channels
  • Angiogenesis Inhibitors
  • Bevacizumab
  • Vascular Endothelial Growth Factor A
  • Clcn2 protein, mouse