Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexus

Lillian I J Byer; Huixin Xu; Maria K Lehtinen

doi:10.1016/j.xpro.2025.103627

Protocol for the dissection, immunostaining, and imaging of whole-mount mouse choroid plexus

STAR Protoc. 2025 Mar 21;6(1):103627. doi: 10.1016/j.xpro.2025.103627. Epub 2025 Feb 27.

Authors

Lillian I J Byer¹, Huixin Xu², Maria K Lehtinen³

Affiliations

¹ Department of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USA. Electronic address: lillian.byer@childrens.harvard.edu.
² Department of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USA.
³ Department of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USA. Electronic address: maria.lehtinen@childrens.harvard.edu.

Abstract

Extracting the choroid plexus (ChP) for histology provides a comprehensive view of the whole tissue and is crucial for understanding its structure and cellular interactions. Here, we present a protocol for dissecting out the lateral, third, and fourth ventricle ChP from mice and the immunostaining process. We also detail procedures for preparing whole-mount ChP tissue and imaging techniques, such as tile scan and Z-stacks. For complete details on the use and execution of this protocol, please refer to Xu et al.¹.

Keywords: cell biology; immunology; microscopy; neuroscience.

MeSH terms

Animals
Choroid Plexus* / anatomy & histology
Choroid Plexus* / cytology
Dissection* / methods
Immunohistochemistry* / methods
Mice