Surface moieties drive the superior protection of curcumin-derived carbon quantum dots against retinal ischemia-reperfusion injury

J Mater Chem B. 2025 Mar 26;13(13):4225-4237. doi: 10.1039/d4tb02364a.

Abstract

Despite the recognized neuroprotective benefits of curcumin, its clinical utility is constrained by poor bioavailability and high cytotoxicity at effective doses. This study evaluates the therapeutic potential of curcumin-derived carbon quantum dots (Cur-CQDs) for retinal protection against ischemia-reperfusion (IR) injury in rats. Cur-CQDs were synthesized via mild pyrolysis at varying temperatures and assessed for efficacy in rat retinal ganglion cells and a model of retinal IR injury. The Cur-CQDs, particularly those synthesized at 150 °C, displayed significant reductions in apoptosis in retinal tissues, as indicated by TUNEL assays, immunofluorescence localization of HIF-α, CD68, BCL-2, and Grp78, and Western blot analysis for HO-1, Grp78, CHOP, caspase 3, and Nrf2. These results suggest that Cur-CQDs not only enhance cell survival and reduce inflammation but also decrease oxidative and endoplasmic reticulum stress markers. Mechanistic insights reveal that Cur-CQDs modulate pathways involved in oxidative stress, apoptosis, and inflammation, specifically through the upregulation of BCL-2 and HO-1 and the downregulation of CHOP, caspase-3, and endoplasmic reticulum stress markers. The identification of cinnamic acid-, anisole-, guaiacol, and ferulic acid-like structures on Cur-CQDs' surfaces may contribute to their superior antioxidative and anti-inflammatory activities. Collectively, these findings position Cur-CQDs as a promising approach for treating retinal IR injuries, enhancing curcumin's bioavailability and therapeutic efficacy, and paving new pathways in ocular neuroprotection research and potential clinical applications.

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Carbon* / chemistry
  • Carbon* / pharmacology
  • Curcumin* / chemistry
  • Curcumin* / pharmacology
  • Male
  • Neuroprotective Agents* / chemistry
  • Neuroprotective Agents* / pharmacology
  • Oxidative Stress / drug effects
  • Quantum Dots* / chemistry
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury* / drug therapy
  • Reperfusion Injury* / metabolism
  • Reperfusion Injury* / pathology
  • Retina / drug effects
  • Surface Properties

Substances

  • Curcumin
  • Carbon
  • Neuroprotective Agents