Growth associated protein 43 (GAP-43) predicts brain amyloidosis in Alzheimer's dementia continuum: an [18 F] AV-45 study

BMC Neurol. 2025 Apr 1;25(1):134. doi: 10.1186/s12883-025-04140-5.

Abstract

Background: Growth-associated protein 43 (GAP-43) is a key protein involved in neuronal growth and synaptic plasticity. Alterations in GAP-43 levels have been associated with Alzheimer's Disease (AD), potentially reflecting synaptic dysfunction. We evaluated the potential of GAP-43 as a biomarker for AD and explored its association with amyloid-beta (Aβ) levels, as well as its correlation with Aβ plaque burden in the brain.

Methods: We screened 1,639 participants from the Alzheimer's Disease Neuroimaging Initiative (ADNI) cohort. A total of 226 individuals met the eligibility criteria and were enrolled. Participants were classified into three groups: 77 cognitively normal (CN) individuals, 111 with mild cognitive impairment (MCI), and 38 with a diagnosis of AD. The associations between cerebrospinal fluid (CSF) GAP-43 levels with other biomarkers as well as [¹⁸F] AV-45 (Florbetapir) PET Standardized Uptake Value Ratios (SUVR) were investigated.

Results: Our findings revealed significantly elevated CSF GAP-43 levels in individuals with AD compared to CN and MCI groups. Furthermore, GAP-43 levels showed a significant positive correlation with tau pathology. Notably, we observed a significant association between GAP-43 and [¹⁸F] Florbetapir PET SUVR in the MCI group, suggesting that GAP-43 may serve as a reliable biomarker in the early stages of AD.

Conclusion: This study provides evidence supporting the role of GAP-43 as a potential biomarker for AD, particularly in relation to predicting the amyloid pathology pattern in the brain in the MCI stage.

Keywords: Alzheimer’s disease; Growth associated protein 43; Mild cognitive impairment; Positron emission tomography.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Alzheimer Disease* / cerebrospinal fluid
  • Alzheimer Disease* / complications
  • Alzheimer Disease* / diagnostic imaging
  • Alzheimer Disease* / metabolism
  • Amyloid beta-Peptides / metabolism
  • Amyloidosis* / cerebrospinal fluid
  • Amyloidosis* / diagnostic imaging
  • Aniline Compounds
  • Biomarkers / cerebrospinal fluid
  • Brain* / diagnostic imaging
  • Brain* / metabolism
  • Cognitive Dysfunction / cerebrospinal fluid
  • Cognitive Dysfunction / diagnostic imaging
  • Cohort Studies
  • Ethylene Glycols
  • Female
  • Fluorine Radioisotopes
  • GAP-43 Protein* / cerebrospinal fluid
  • Humans
  • Male
  • Middle Aged
  • Positron-Emission Tomography

Substances

  • GAP-43 Protein
  • Biomarkers
  • Aniline Compounds
  • Ethylene Glycols
  • Fluorine Radioisotopes
  • Amyloid beta-Peptides
  • florbetapir