Green fabrication of antimicrobial film for food preservation by polysaccharides from Poria Cocos

Int J Biol Macromol. 2025 May;309(Pt 4):143131. doi: 10.1016/j.ijbiomac.2025.143131. Epub 2025 Apr 12.

Abstract

Recently, polysaccharide-based film materials have attracting widespread attentions due to their safety, biodegradability, and film-forming properties. However, the limited UV resistance, antibacterial and mechanical properties of polysaccharides severely restrict their applications in food packaging. This work developed a biocomposite film (PGF@Ag) consited of a natural β-glucan from Poria Cocos (PCPA), in-situ synthesized AgNPs and glycerol, showing good antibacterial and antioxidant properties as well as biocompatibility and mechanical properties. Poria Cocos is an edible traditional Chinese medicine, and its residue is rich in polysaccharide. In this system, the large amont of hydrogen groups on PCPA help fast reduce Ag+ to Ag0 in alkalescent solution and the triple-helix conformation of PCPA hinder the aggregation of AgNPs, leading to the ultra small size with 9.0-12.3 nm as well as better ABTS radical scavenging rates of 93.30 ± 0.13 %. Additionally, the hydrogen bonding interactions between glycerol and polysaccharides significantly enhance the mechanical properties of the PGF@Ag films. The tensile strength is measured at 5.94 ± 0.5 MPa, while the elongation at break reaches 128.77 ± 12.45 %, and the Young's modulus is recorded at 15.75 ± 1.91 MPa. Finally, the resulting films exhibited excellent preservation effects on grapes, as reduced weight loss reduction of 14 %, and hardness reduction of approximately 5.2 % over 12 days. This work provides a new strategy of the sustainable construction of antibacterial films, showing great potentials in food preservation and packaging applications.

Keywords: AgNPs; Food preservation; Glycerol; Poria Cocos; β-Glucan.

MeSH terms

  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / pharmacology
  • Anti-Infective Agents* / chemistry
  • Anti-Infective Agents* / pharmacology
  • Antioxidants / chemistry
  • Antioxidants / pharmacology
  • Food Packaging
  • Food Preservation* / methods
  • Green Chemistry Technology*
  • Metal Nanoparticles / chemistry
  • Polysaccharides* / chemistry
  • Polysaccharides* / pharmacology
  • Silver / chemistry
  • Tensile Strength
  • Wolfiporia* / chemistry

Substances

  • Polysaccharides
  • Anti-Bacterial Agents
  • Antioxidants
  • Silver
  • Anti-Infective Agents