Loss of HKT1;5D via a spontaneous terminal deletion elevates leaf sodium in bread wheat, but is independent of yield or salinity tolerance

Yusuf Genc; Ute Baumann; Judy Cheong; Julian Taylor; Judith Atieno; James Walter; Tristan Coram; Julie E Hayes; Tim Sutton

doi:10.1093/jxb/eraf213

Loss of HKT1;5D via a spontaneous terminal deletion elevates leaf sodium in bread wheat, but is independent of yield or salinity tolerance

J Exp Bot. 2025 May 19:eraf213. doi: 10.1093/jxb/eraf213. Online ahead of print.

Authors

Yusuf Genc^{1

2}, Ute Baumann², Judy Cheong¹, Julian Taylor², Judith Atieno^{1

2}, James Walter³, Tristan Coram³, Julie E Hayes², Tim Sutton^{1

2}

Affiliations

¹ South Australian Research and Development Institute, Plant Genomics Centre, Waite Campus, Glen Osmond, SA, Australia.
² Waite Research Institute, School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, Glen Osmond, SA, Australia.
³ Australian Grain Technologies Pty Ltd, 20 Leitch Road, Roseworthy, SA, Australia.

PMID: 40384338
DOI: 10.1093/jxb/eraf213

Abstract

Soil salinity is a significant constraint to global wheat production, and breeding for salinity tolerance offers a sustainable solution. MW#293 is a recently developed bread wheat line, notable for both its high salinity tolerance and unusually high leaf sodium (Na⁺) accumulation. However, the association between high Na⁺ accumulation and salinity tolerance in this line was unknown. Using genetic, genomic, physiological, and agronomic approaches, we identified and delineated a terminal deletion on chromosome 4D encompassing the major Na⁺ transporter gene TaHKT1;5D and determined this as the cause of the elevated leaf Na⁺ in MW#293. In a Mace x MW#293 segregating population, leaf Na+ and shoot growth measured under salinity were shown to be under separate genetic control. Field trials were conducted in three environments ranging from slightly to moderately saline. Despite the deletion encompassing at least 286 genes, we observed neither a positive nor a negative influence on grain yield. These results suggest that TaHKT1;5D is not a critical determinant of salinity tolerance in MW#293 and related bread wheat germplasm, but that other mechanisms are responsible.

Keywords: HKT1;5D; chromosome deletion; salinity; salinity tolerance; sodium transporter; wheat.

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