Isolation of Extracellular Vesicle Subtypes from Primary Osteoblast Cultures

Bethan K Davies; Saida Mebarek; Owen G Davies; Isabel R Orriss

doi:10.1007/978-1-0716-4306-8_8

Isolation of Extracellular Vesicle Subtypes from Primary Osteoblast Cultures

Methods Mol Biol. 2025:2885:143-157. doi: 10.1007/978-1-0716-4306-8_8.

Authors

Bethan K Davies¹, Saida Mebarek², Owen G Davies³, Isabel R Orriss⁴

Affiliations

¹ Department of Clinical and Experimental Endocrinology, KU Leuven, Leuven, Belgium.
² Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université de Lyon, Lyon, France.
³ School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK.
⁴ Department of Comparative Biomedical Sciences, Royal Veterinary College, London, UK. iorriss@rvc.ac.uk.

PMID: 40448760
DOI: 10.1007/978-1-0716-4306-8_8

Abstract

This chapter describes the isolation and characterization of extracellular vesicles (EVs) isolated from primary rodent osteoblasts and extracellular matrix. The key advantages of this assay are that it allows the isolation of EVs from the conditioned media of primary osteoblasts at varying stages of differentiation and subsequent characterization prior to downstream applications.

Keywords: Exosomes; Extracellular vesicles; Matrix vesicles; Microvesicles; Osteoblast.

MeSH terms

Animals
Cell Differentiation
Cells, Cultured
Culture Media, Conditioned
Extracellular Matrix / metabolism
Extracellular Vesicles* / metabolism
Mice
Osteoblasts* / cytology
Osteoblasts* / metabolism
Primary Cell Culture / methods
Rats

Substances

Culture Media, Conditioned