Fecal metabolite profiling identifies critically ill patients with increased 30-day mortality

Sci Adv. 2025 Jun 6;11(23):eadt1466. doi: 10.1126/sciadv.adt1466. Epub 2025 Jun 4.

Abstract

Critically ill patients admitted to the medical intensive care unit (MICU) have reduced intestinal microbiota diversity and altered microbiome-associated metabolite concentrations. Metabolites produced by the gut microbiota have been associated with survival of patients receiving complex medical treatments and thus might represent a treatable trait to improve clinical outcomes. We prospectively collected fecal specimens, defined microbiome compositions by shotgun metagenomic sequencing, and quantified microbiota-derived fecal metabolites by mass spectrometry from 196 critically ill patients admitted to the MICU for non-COVID-19 respiratory failure or shock to correlate microbiota features and metabolites with 30-day mortality. Microbiota compositions of the first fecal sample after MICU admission did not independently associate with 30-day mortality. We developed a metabolic dysbiosis score (MDS) that uses fecal concentrations of 13 microbiota-derived metabolites, which predicted 30-day mortality independent of known confounders. The MDS complements existing tools to identify patients at high risk of mortality by incorporating potentially modifiable, microbiome-related, independent contributors to host resilience.

MeSH terms

  • Aged
  • Critical Illness* / mortality
  • Dysbiosis / microbiology
  • Dysbiosis / mortality
  • Feces* / chemistry
  • Feces* / microbiology
  • Female
  • Gastrointestinal Microbiome*
  • Humans
  • Intensive Care Units
  • Male
  • Metabolome*
  • Metabolomics / methods
  • Middle Aged
  • Prospective Studies