Goat milk oligosaccharides improve growth and gut microbiota composition in stunted mice

Abstract

The gut microbiota plays a crucial role in the growth and development of the host. Prebiotics that modulate the gut microbiota are considered potential agents for regulating host growth, development, and bone health. However, the effects of goat milk oligosaccharides (GMO) to promote growth and development remains unclear. Here, we established a mouse model of stunted growth by feeding a low-fat, low-protein diet, followed by intervention with low, medium, and high doses of GMO. Our findings demonstrated that GMO increased body weight, body length, lean mass, fat mass, organ weight, tibial length, and femoral length in mice. Micro-CT analysis revealed that GMO promoted bone mass, enhanced trabecular number, and decreased trabecular distance, but had no significant effect on trabecular thickness. GMO promoted bone formation by increasing P1NP and Osteocalcin contents, and inhibited bone resorption by reducing CTX-I and TRACP contents. Furthermore, GMO also upregulated the expression levels of insulin-like growth factor 1 (IGF-1), insulin-like growth factor 1 receptor (IGF-1R), and AKT1 in mice. Gut microbiota analysis revealed that GMO increased microbial diversity and promoted the relative abundance of Bacteroidota, including Bacteroides uniformis, Bacteroides xylanisolvens, Bacteroides sp. HF162, and Bacteroides fragilis. Additionally, we observed a dose-dependent effect of GMO on growth and development, where higher doses resulted in greater effects. Overall, our results suggest that a high dose of GMO enhance growth performance and alters gut microbiota composition in stunted mice.

Keywords: Bone; Goat milk oligosaccharides; Growth and development; Gut microbiota.