Impact of Poly-L-Lactic Acid Reconstitution on the Neocollagenesis Profile

Janaína Motta Guerra; Maria Carolina Portela Albuquerque; Clarice Lioba de Araújo; Júlia Gonçalves Montenegro; Diana Araújo Cunha; Fabrício Bitu Sousa; Paulo Goberlânio de Barros Filho; Átila Vinícius Vitor Nobre

doi:10.1007/s00266-025-04970-1

Impact of Poly-L-Lactic Acid Reconstitution on the Neocollagenesis Profile

Aesthetic Plast Surg. 2025 Jun 5. doi: 10.1007/s00266-025-04970-1. Online ahead of print.

Authors

Janaína Motta Guerra¹, Maria Carolina Portela Albuquerque¹, Clarice Lioba de Araújo¹, Júlia Gonçalves Montenegro¹, Diana Araújo Cunha¹, Fabrício Bitu Sousa¹, Paulo Goberlânio de Barros Filho¹, Átila Vinícius Vitor Nobre²

Affiliations

¹ Department of Dentistry, Christus University Center, João Adolfo Gurgel Street, 133, Cocó, Fortaleza, CE, 60192-345, Brazil.
² Department of Dentistry, Christus University Center, João Adolfo Gurgel Street, 133, Cocó, Fortaleza, CE, 60192-345, Brazil. atilavnobre@hotmail.com.

PMID: 40473786
DOI: 10.1007/s00266-025-04970-1

Abstract

Purpose: The objective of the present work was to evaluate the percentage of neocollagenesis induction of the Sculptra^® in different forms of reconstitution.

Methods: For this, 150 male Swiss mice were used to which saline solution or reconstituted Sculptra^® were applied to the back subcutaneous tissue immediately before application (0 h), 2, 24 or 72 h before administration and were euthanized after 15, 30 and 60 days of administration of the material in a number of 10/group/day. Their backs were removed for histological analysis and immunoexpression for alpha-smooth muscle actin (α-SMA). The data was statistically analyzed (p < 0.05).

Results: After 30 days of administration, the Sculptra^® 72 h group showed greater collagen formation than the control (p = 0.016) and 0h groups (p = 0.023). After 60 days, the Sculptra^® 24 and 72 h groups also showed greater collagen formation than the control (p = 0.025) and 0 h groups ( p = 0.039). After 30 days of administration, the Sculptra^® 24 and 72 h groups showed higher immunoexpression for α-SMA (p = 0.001), as well as after 60 days (p < 0.001).

Conclusions: We can conclude that Sculptra^® reconstituted 2, 24 and 72 h before its application has the potential to induce collagen after 60 days. Furthermore, there is greater expression of α-SMA after reconstitution 24 and 72 h before Sculptra^® injection, after 30 and 60 days. No level of evidence is needed for Basic Science, Animal Study, Cadaver Study, and Experimental Study Articles.

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Keywords: Collagen; Dermal fillers; Poly-L-lactic acid.