Growth-differentiation factor 15 (GDF-15) is associated with arterial stiffness and impaired endothelial function with mediation by albuminuria in the Singapore study of macroangiopathy and microvascular reactivity in type 2 diabetes (SMART2D) cohort

Endocrine. 2025 Jun 7. doi: 10.1007/s12020-025-04283-y. Online ahead of print.

Abstract

Objective: Growth differentiation factor-15 (GDF-15) is a stress-reactive cytokine which is implicated in the pathogenesis of several diabetic complications. GDF-15 may induce endothelial dysfunction which is a determinant of arterial stiffness. We aimed to study the association between GDF-15 with pulse wave velocity (PWV) and endothelial function in patients with T2D, and the potential mediating role of albuminuria.

Methods: This was a prospective cohort study of 1784 patients with T2D. Baseline plasma GDF-15 was measured using multiplex immunoassay. Carotid-femoral PWV was measured using applanation method. We used laser Doppler imaging with iontophoresis to measure forearm endothelium-independent vasodilation (EIV) and endothelium-dependent vasodilation (EDV).

Results: The mean age of patients was 57.0 ± 10.8 years. Cross-sectionally, GDF-15 was associated with higher PWV and lower EDV at baseline. Baseline GDF-15 was also associated with higher PWV and lower EIV at follow-up with adjusted coefficients 0.33 (95%CI 0.15-0.52; p < 0.001) and -2.09 (95%CI -4.14--0.05; p = 0.045) respectively. Albuminuria mediated 29.5% and 44.9% of the association between baseline GDF-15 with follow-up PWV and EDV respectively.

Conclusion: Baseline GDF-15 was independently associated with higher PWV and lower EDV over time, with mediation by albuminuria in patients with T2D. GDF-15 is a potential biomarker of arterial stiffness and impaired endothelial function in T2D.

Keywords: Arterial stiffness; Diabetes; Endothelial function; Growth-differentiation factor 15.