Role of the CEACAM7-JAK2/STAT3-BST2 Axis in the migration of nasopharyngeal carcinoma cells

Ju-Pi Li; Chiao-Wen Lin; Yen-Ting Lu; Cheng-Chen Huang; Yu-Ting Ho; Chung-Han Hsin; Shun-Fa Yang

doi:10.1016/j.bbadis.2025.167948

Role of the CEACAM7-JAK2/STAT3-BST2 Axis in the migration of nasopharyngeal carcinoma cells

Biochim Biophys Acta Mol Basis Dis. 2025 Jun 5;1871(7):167948. doi: 10.1016/j.bbadis.2025.167948. Online ahead of print.

Authors

Ju-Pi Li¹, Chiao-Wen Lin², Yen-Ting Lu³, Cheng-Chen Huang⁴, Yu-Ting Ho⁵, Chung-Han Hsin⁶, Shun-Fa Yang⁷

Affiliations

¹ School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Pediatrics, Chung Shan Medical University Hospital, Taichung, Taiwan.
² Institute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan.
³ School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan; Department of Otolaryngology, St. Martin De Porres Hospital, Chiayi, Taiwan.
⁴ School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan.
⁵ Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan.
⁶ School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan. Electronic address: hsin@csmu.edu.tw.
⁷ Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan. Electronic address: ysf@csmu.edu.tw.

PMID: 40482926
DOI: 10.1016/j.bbadis.2025.167948

Abstract

Objective: Nasopharyngeal carcinoma (NPC) is a rare malignancy of the head and neck, with recurrence and distant metastasis being the primary causes of mortality among affected patients. Carcinoembryonic antigen cellular adhesion molecules (CEACAMs) are implicated in various physiological and pathophysiological processes. However, the role of CEACAM7 in NPC progression remains unclear. This study investigated the influence of CEACAM7 on NPC progression.

Methods: Cell migration and invasion ability were conducted using a Boyden chamber assay. Regulation of signaling pathways was evaluated by immunoblotting.

Results: The findings revealed that CEACAM7 is highly expressed in human NPC tissues, with particularly elevated levels observed in malignant tissues and high-grade squamous cell carcinoma tissues. Furthermore, CEACAM7 was demonstrated to regulate cell migration and invasion in human NPC cells but did not influence cell proliferation. Moreover, CEACAM7 enhanced cell motility through the activation of the JAK2/STAT3/BST2 signaling pathway. Notably, cotreatment with WP1066, a JAK2/STAT3 inhibitor, suppressed CEACAM7-induced cell migratory ability and downregulated BST2 expression in NPC cells.

Conclusions: These findings indicate that the CEACAM7/JAK2/STAT3/BST2 axis plays a crucial role in promoting NPC cell migration and underscore its potential as a therapeutic target for mitigating NPC metastasis.

Keywords: BST2; CEACAM7; Cancer metastasis; JAK2; Nasopharyngeal carcinoma; STAT3.