Near-infrared chemiluminescent probes for monitoring leucine aminopeptidase activity

Haoyun Xue; Jing Yang; Xiuyan Zhao; Zhantao Zhang; Ming Liu; Zhiqian Guo

doi:10.1039/d5cc02329g

Near-infrared chemiluminescent probes for monitoring leucine aminopeptidase activity

Chem Commun (Camb). 2025 Jul 3;61(55):10107-10110. doi: 10.1039/d5cc02329g.

Authors

Haoyun Xue¹, Jing Yang¹, Xiuyan Zhao¹, Zhantao Zhang², Ming Liu³, Zhiqian Guo¹

Affiliations

¹ Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa, Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai 200237, China. guozq@ecust.edu.cn.
² Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000, China. 16645614@qq.com.
³ School of Perfume and Aroma Technology, Shanghai-Institute of Technology, Shanghai 20141, China. mingliu@sit.edu.cn.

PMID: 40497741
DOI: 10.1039/d5cc02329g

Abstract

Near-infrared (NIR) chemiluminescent probes offer high sensitivity for biological sensing applications. Herein, we present two NIR chemiluminescent probes for leucine aminopeptidase (LAP) detection, designed by covalently linking the dicyanomethylene-4H-pyran (DCM) fluorophore to Schaap's dioxetane scaffold. The impact of substitution position on chemiluminescence kinetics and enzymatic responsiveness revealed that only the para-substituted probe with minimal steric hindrance enables efficient in vitro and in vivo imaging of LAP activity.

MeSH terms

Animals
Fluorescent Dyes* / chemical synthesis
Fluorescent Dyes* / chemistry
Humans
Leucyl Aminopeptidase* / analysis
Leucyl Aminopeptidase* / metabolism
Luminescent Measurements
Mice
Molecular Structure
Optical Imaging

Substances

Leucyl Aminopeptidase
Fluorescent Dyes