MicroRNA 150 (miR-150) and exosomal miR-150 are the circulating and potential cardiovascular therapeutic targets. The downregulation of macrophage-derived exosomal miR-150-5p and overexpression of resistin affect the diabetic pathogenesis. Catalpol, an iridoid glucoside, has anti-diabetic, cardiovascular protective, and anti-inflammatory effects. The aim of this study was to investigate the cardioprotective effects of catalpol due to enhanced macrophage-derived exosomal miR-150-5p under hyperglycemia stress in vitro and in vivo. Catalpol and macrophage-derived exosomal miR-150-5p were the treatments in these diabetic atherosclerotic models. Luciferase activity assay, immunohistochemistry, and immunofluorescent analyses were employed. The data revealed that hyperglycemic stress suppressed the exosomal miR-150-5p levels in high glucose (25 mM)-cultured mouse macrophages, however, increased their resistin levels. Treatment with catalpol, pretreated-catalpol-macrophage-derived exosomes augmented the levels of exosomal miR-150-5p in mouse macrophage cultures under hyperglycemic stress in vitro, ameliorated resistin presentation simultaneously. Treatment with pretreated-catalpol-macrophage-derived exosomes, catalpol (5 mg/kg), and exosomal miR-150-5p dominant considerably reduced the neointimal sizes by 37.6 %, 24.8 % and 39.7 %, respectively, after 14 days of balloon injury in vivo. Our novelty of findings indicate catalpol ameliorates resistin and neointimal hyperplasia formation under hyperglycemia stress by mediating the macrophage-derived exosomal miR-150-5p. Catalpol has cardioprotective effects to modulate the ell-to-cell communication in a hyperglycemic microenvironment.
Keywords: Catalpol; Diabetes; Exosome; Neointima; Resistin; microRNA 150-5p.
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