Strontium isotopes (87Sr/86Sr) are increasingly used as a provenance tool in multiple disciplines. Application to biological materials requires knowledge of the variation in bioavailable 87Sr/86Sr across the landscape, potentially in the form of an isoscape (a quantitative model of spatial isotopic variability). This paper summarizes and provides advice on our current understanding of the main concerns in creating and interpreting isoscapes of bioavailable 87Sr/86Sr. Isoscape creation approaches include domain mapping, geostatistical contour mapping and machine learning, the last becoming more readily achievable with the availability of software packages. It is critically important to develop isoscapes at a resolution appropriate for addressing the research questions. Choice of sample materials depends on the research questions and availability: plants or fauna with small ranges are favoured, with some analytes (snails, soil leachates) posing challenges. Interpreting 87Sr/86Sr in biological tissues requires considering Sr metabolism and the timing of tissue formation, thus far underappreciated. The numerous sources of error involved in developing and applying isoscapes must be recognized to avoid over-interpreting data and spurious provenance precision. We hope this paper will help researchers investigating provenance, mobility, landscape use and migration to develop the most appropriate isoscapes for their purposes, and possible future use by others.
Keywords: bioavailable 87Sr/86Sr; ecology; geochemistry; geolocation; geostatistics; isotope mapping.
© 2025 The Authors.