Alkaloids from the tubers of Pinellia pedatisecta

Xu Chen; Yan-Gang Cao; Feng-Xiao Hao; Yan-Ling Liu; Fang-Ge Chi; Ying Niu; Di Lu; Bing-Xian Zhao; Lan Chen; Xiao-Ke Zheng; Wei-Sheng Feng

doi:10.1016/j.phytochem.2025.114597

Alkaloids from the tubers of Pinellia pedatisecta

Phytochemistry. 2025 Jun 19:239:114597. doi: 10.1016/j.phytochem.2025.114597. Online ahead of print.

Authors

Affiliations

¹ School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province China, Zhengzhou, 450046, China.
² School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province China, Zhengzhou, 450046, China. Electronic address: zhengxk.2006@163.com.
³ School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province China, Zhengzhou, 450046, China; Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of PR China, Zhengzhou, 450046, China. Electronic address: fwsh@hactcm.edu.cn.

PMID: 40543641
DOI: 10.1016/j.phytochem.2025.114597

Abstract

Nine undescribed alkaloids (1, 3, 4, 7, 8 and 10-13) and four undescribed natural products (2, 5, 6 and 9), together with eight known components, were isolated from the tubers of Pinellia pedatisecta Schott. Their structures were elucidated based on spectroscopic data, Rh₂(OCOCF₃)₄-induced ECD spectral analysis, DP4+ analysis, ECD analysis and interpretation of their optical rotation data. The protective effects of all compounds against Aβ_25-35-induced PC-12 cell injury were tested by MTT method, and the results indicated that compounds 1, 2, 5-10 and 16-20 exhibited obviously protective effects. In addition, the action mechanisms of compounds 2 and 5 were investigated by flow cytometry and cellular immunofluorescence, and the results indicated that compounds 2 and 5 ameliorated Aβ_25-35-induced PC-12 cell injury via the NMDAR/p-CamK II/PKG signaling pathway. This study provided experimental basis for researching the chemical composition of P. pedatisecta and developing natural medicines for the treatment of Alzheimer's disease in the future.

Keywords: Alkaloids; Alzheimer's disease; Araceae; Pinellia pedatisecta.