Effects of miR-200b-3p and miR-424-5p regulating notch signaling pathway on atherosclerosis induced by PM2.5in vitro

Tianyang Zhao; Xu Li; Zhili Ge; Jingjing Shi; Tianyou Wang; Jiaxin Zhang; Xinyu Zhang; Huibin Jiang; Liting Zhou; Lin Ye

doi:10.1016/j.tox.2025.154216

Effects of miR-200b-3p and miR-424-5p regulating notch signaling pathway on atherosclerosis induced by PM_2.5in vitro

Toxicology. 2025 Jun 17:517:154216. doi: 10.1016/j.tox.2025.154216. Online ahead of print.

Authors

Tianyang Zhao¹, Xu Li¹, Zhili Ge¹, Jingjing Shi¹, Tianyou Wang¹, Jiaxin Zhang¹, Xinyu Zhang¹, Huibin Jiang¹, Liting Zhou², Lin Ye³

Affiliations

¹ Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China.
² Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China. Electronic address: zhoulttg@163.com.
³ Department of Occupational and Environmental Health, School of Public Health, Jilin University, Changchun, China. Electronic address: jlyelin@163.com.

PMID: 40553880
DOI: 10.1016/j.tox.2025.154216

Abstract

Atmospheric particulate matter (PM) is one of the main pollutants of air pollution, which adsorbs several harmful organic substances, heavy metals, bacteria, and viruses. Epidemiological research has demonstrated that PM_2.5 exposure might alter subclinical markers of atherosclerosis while the mechanism is unclear. In the current study, macrophages and foam cells that were differentiated from THP-1 cells were exposed to PM_2.5 (0, 25, 50, and 100 μg/mL). PM_2.5 exposure reduce the expression of miR-200b-3p and miR-424-5p, thereby regulating the Notch signaling pathway in macrophages and foam cells, which affects the expression of lipid metabolism-related genes (ABCA1, LDLR, SR-A, SR-BI and CD36) and aggravates the dysfunction of lipid metabolism and leads to atherosclerosis. The study provides new ideas for the prevention and treatment of atherosclerosis.

Keywords: Fine particulate matters; MiR-200b-3p and miR-424–5p; Notch signaling pathway.