The WRKY transcription factor family is a significant family of plant transcription factors (TFs). Plant growth and development are often influenced by abiotic factors, such as salinity and low temperature. Numerous studies have demonstrated that WRKY TFs primarily influence plant responses to adversity. However, there are few studies on the role of WRKY genes in the stress responses of Malus baccata (L.) Borkh. We cloned the MbWRKY33 gene from Malus baccata for this research, and its roles in salt stress tolerance were analyzed. Phylogenetic tree analysis revealed that MbWRKY33 and PbWRKY33 have the highest homology. Subcellular localization revealed that MbWRKY33 was located within the nucleus. An analysis of tissue-specific expression showed that MbWRKY33 had relatively high expression levels in young leaves and roots. Moreover, Arabidopsis thaliana plants overexpressing MbWRKY33 exhibited stronger resistance to salt stress compared with the wild type (WT) and the unloaded line empty vector (UL). Under the treatment of 200 mM NaCl, transgenic Arabidopsis thaliana plants exhibited significantly higher activities of antioxidant enzymes like superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) than the control. In contrast, the WT and the UL lines had elevated levels of malondialdehyde (MDA) and reactive oxygen species (ROS). In addition, MbWRKY33 elevates transgenic plant resistance to salt stress by regulating the expression levels of AtNHX1, AtSOS1, AtSOS3, AtNCED3, AtSnRK2, and AtRD29a. Results indicated that MbWRKY33 in Malus might be linked to high-salinity stress responses, laying a foundation for understanding WRKY TFs' reaction to such stress.
Keywords: Malus baccata; MbWRKY33; genetic transformation; high-salinity stress; transcriptional regulation.