Background and Objectives: Complications in wound healing present significant challenges in clinical settings. While platelet-rich plasma from human sources has been extensively used to aid wound recovery, allogeneic or xenogeneic platelet-derived products remain in the research phase. This study aimed to assess both the immunogenicity and therapeutic potential of xenogeneic porcine platelet lysate (pPL) in wound healing, using a rat model. Materials and Methods: Porcine platelet lysates with undetectable levels of antigens, including blood cells and complement factors, were engineered. Rat models simulating wound conditions were employed to investigate the effects of xenogeneic pPL on injured skin tissues. Histological assessments, including re-epithelialization, angiogenesis, and inflammatory cell response, were comprehensively conducted to evaluate the healing process. Results: The application of xenogeneic pPL on rat skin incisions significantly expedited the wound healing process. No rejection reaction was observed. Histological examinations of the xenogeneic pPL-treated wounds revealed enhanced re-epithelialization and angiogenesis compared to the wounds in control groups. Conclusions: These findings support the clinical promise of xenogeneic pPL as a feasible and effective agent for wound repair and tissue regeneration. This study suggests that its potential application in in vivo regeneration appears viable and promising.
Keywords: in vivo study; porcine platelet lysate; wound healing; xenogeneic.