Coamorphous System of Vitamin B2 and Chlorogenic Acid: Enhanced Physicochemical Property and Synergistic Anti-Inflammatory Potential

Xin Chen; Jia Deng; Jiangying Li; Ningbo Pang; Zhe Wang; Qian Dai; Hailu Zhang

doi:10.1021/acs.molpharmaceut.5c00858

Coamorphous System of Vitamin B2 and Chlorogenic Acid: Enhanced Physicochemical Property and Synergistic Anti-Inflammatory Potential

Mol Pharm. 2025 Jun 27. doi: 10.1021/acs.molpharmaceut.5c00858. Online ahead of print.

Authors

Xin Chen¹, Jia Deng², Jiangying Li³, Ningbo Pang¹, Zhe Wang⁴, Qian Dai², Hailu Zhang⁵

Affiliations

¹ Innovation Centre for Science and Technology, North Sichuan Medical College, Nanchong, Sichuan 637000, P. R. China.
² Clinical Laboratory, Institute of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637001, P. R. China.
³ Laboratory of Pharmaceutical Solid-State Chemistry, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, P. R. China.
⁴ Hunan Provincial Key Laboratory of Basic and Clinical Pharmacological Research of Gastrointestinal Cancer, Department of Pharmacy, Institute of Pharmacy and Pharmacology, the Second Affiliated Hospital, University of South China, Hengyang, Hunan 421001, P. R. China.
⁵ Interdisciplinary Institute of NMR and Molecular Sciences, Key Laboratory of Hubei Province for Coal Conversion and New Carbon Materials, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, P. R. China.

PMID: 40577621
DOI: 10.1021/acs.molpharmaceut.5c00858

Abstract

Vitamin B2 (VB2), a functional ingredient found in various foods and herbal medicines, is crucial for metabolic processes, with its deficiency linked to inflammatory diseases. However, the poor water solubility of VB2 hampers its development and application. This study employed chlorogenic acid (CGA) to create a coamorphous system with VB2 to improve its physicochemical properties. The coamorphous VB2-CGA was initially confirmed using powder X-ray diffraction (PXRD) and modulated temperature differential scanning calorimetry (mDSC). Fourier-transform infrared spectroscopy (FTIR) and molecular dynamics (MD) simulations revealed potential molecular interactions within the system. The water solubility and physical stability of the VB2-CGA coamorphous system were further evaluated. Notably, the VB2-CGA system exhibited a 2.7-fold solubility increase compared to crystalline VB2 and maintained physical stability for at least one year under dry conditions. Additionally, the potential multitarget synergistic anti-inflammatory effects of the VB2-CGA coamorphous system were also disclosed using network pharmacological analysis.

Keywords: Vitamin B2; chlorogenic acid; coamorphous system; multitarget synergistic anti-inflammatory effect; solubility.