Novel Supplementation of Fe3O4-Doped Green Carbonized Nanoparticles on Hydrogenases Genes and Microbial Biodiversity for Enhancing Biohydrogen Yield in Dark Fermentation Microbial Electrohydrogenesis Cells

Hikmatullah Ahmadi; Anam Jalil; Sohail Khan; Irfan Ali Phulpoto; Zhang Chengyu; Zhisheng Yu

doi:10.1093/jimb/kuaf016

Novel Supplementation of Fe3O4-Doped Green Carbonized Nanoparticles on Hydrogenases Genes and Microbial Biodiversity for Enhancing Biohydrogen Yield in Dark Fermentation Microbial Electrohydrogenesis Cells

J Ind Microbiol Biotechnol. 2025 Jun 19:kuaf016. doi: 10.1093/jimb/kuaf016. Online ahead of print.

Authors

Hikmatullah Ahmadi^{1

2

3

4}, Anam Jalil^{1

3

4}, Sohail Khan^{1

3}, Irfan Ali Phulpoto^{1

3}, Zhang Chengyu^{1

3}, Zhisheng Yu^{1

2

3

4}

Affiliations

¹ College of Resources and Environment, University of Chinese Academy of Sciences, 19 A Yuquan Road, Beijing, 100049, PR China.
² Binzhou Institute of Technology, Weiqiao-UCAS Science and Technology Park, Binzhou City 256606, Shandong Province, PR China.
³ RCEES-IMCAS-UCAS Joint-Laboratory of Microbial Technology for Environmental Science, Beijing 100085, P.R. China.
⁴ University of Chinese Academy of Sciences, Beijing 100049, P.R. China.

PMID: 40577822
DOI: 10.1093/jimb/kuaf016

Abstract

Achieving high-purity biohydrogen (Bio-H₂) production necessitates the suppression of hydrogenotrophic methanogens, as their activity can impede hydrogen yield. Various inoculum pretreatments have been employed to suppress methane-producing Microorganisms; however, these methods can negatively impact the enzymatic activity of hydrogen-producing microorganisms, thereby reducing hydrogen production. To address this challenge, this research investigates a novel approach to enhance Bio-H₂ production by activating microbial enzymes using magnetite Fe₃O₄-doped carbonized nanoparticles (NPs) derived from vegetable leaves (VLCFe₃O₄-NPs) within a coupled dark fermentation-microbial Electrohydrogenesis system. Characterization results revealed that VLCFe₃O₄-NPs exhibited cubic and spherical morphologies, with a small diameter of 1±100 nm and a mean crystallite size of 38.1 nm, indicating high purity. Fermentation tests investigated the impact of different nanoparticle dosages on Bio-H₂ generation, hydrogenase gene expression (Fe-Fe and Ni-Fe), and microbial biodiversity. Bio-H₂ production significantly improved with 500 mg/L VLCFe₃O₄-NPs, yielding 1.2-fold more than the control group, while even a low dose of 25 mg/L resulted in a 0.22-fold increase. Relative gene expression analysis using qPCR and the 2-ΔΔCT method demonstrated a 30-fold increase in Cbei 1773 (Fe-Fe hydrogenase) and a 23-fold increase in hucL (Ni-Fe hydrogenase) gene expression, along with an increase in 16S rDNA. Additionally, the abundance of biohydrogen-producing bacteria, Clostridium_sensu_stricto_1 and Clostridium_sensu_stricto_11, increased by 14.3% and 11.1%, respectively, compared to 4.9% and 3.9% in the control group. This research indicates that VLCFe₃O₄-NPs offer an eco-friendly solution for boosting biohydrogen production within DF-MECs systems, thereby supporting sustainable bioenergy generation.

Keywords: Biohydrogen; DF-MECs; Hydrogenase Genes; Microbial Biodiversity; VLCFe3O4 Nanoparticles.