Evaluating the effectiveness of COVID-19 vaccines during a period of Omicron variant predominance among Bangladeshi population: A test-negative design measurement

Hum Vaccin Immunother. 2025 Dec;21(1):2518644. doi: 10.1080/21645515.2025.2518644. Epub 2025 Jun 28.

Abstract

The study was conducted to estimate the protective effectiveness (PE) of complete primary or booster dose regimens of COVID-19 vaccines deployed in Bangladesh. The study was conducted in four hospitals in Dhaka between December 30, 2021, and August 31, 2022 following a test-negative design. Patients aged ≥18 years attended with COVID-like symptoms were enrolled and tested for RT-PCR. Test-negative controls were matched to confirmed cases at a 1:1 ratio considering site, date, and age groups. Conditional logistic regression was used to estimate the PE considering the association between receipt of complete primary with or without a booster regimen and development of COVID-19 disease symptoms. Whole genome sequencing (WGS) was carried out to confirm the variants. RT-PCR positive 847 cases were matched to 847 controls. WGS of strains revealed 6% to be the Delta variant and 94% was Omicron variant. The PE conferred by receipt of complete primary regimen with or without booster dose of any vaccine revealed no significant protection (15%, 95% CI: -11 to 36, p = .23) against any COVID-19 disease or severe disease (14%, 95%CI: -23 to 39, p = .42). However, there was a protective association between receipt of complete primary regimen with or without booster dose of one mRNA vaccine (Pfizer-BioNTech) against any COVID-19 disease (88% (95% CI: 26 to 98, p = .023)) for the first 90 days. The analysis suggested little vaccine effectiveness during Omicron surge, with the possible exception of one mRNA-vaccine 90 days after dosing.

Keywords: Bangladesh; COVID-19 vaccines; omicron variant; test-negative design (TND) vaccine effectiveness.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Bangladesh / epidemiology
  • COVID-19 Vaccines* / administration & dosage
  • COVID-19 Vaccines* / immunology
  • COVID-19* / epidemiology
  • COVID-19* / immunology
  • COVID-19* / prevention & control
  • COVID-19* / virology
  • Case-Control Studies
  • Female
  • Humans
  • Immunization, Secondary
  • Male
  • Middle Aged
  • SARS-CoV-2* / genetics
  • SARS-CoV-2* / immunology
  • Vaccine Efficacy*
  • Whole Genome Sequencing
  • Young Adult

Substances

  • COVID-19 Vaccines

Supplementary concepts

  • SARS-CoV-2 variants