Power of Short Tandem Repeat (STR) Analysis for Analysis of Genetic Differences and Genotype Identification

Anthony Torres; Reginald Gaudino

doi:10.1007/978-1-0716-4642-7_13

Power of Short Tandem Repeat (STR) Analysis for Analysis of Genetic Differences and Genotype Identification

Methods Mol Biol. 2025:2943:157-166. doi: 10.1007/978-1-0716-4642-7_13.

Authors

Anthony Torres¹, Reginald Gaudino^{2

1}

Affiliations

¹ Cannabis Research Institute, Discovery Partners Institute, University of Illinois System, Chicago, IL, USA.
² Front Range Biosciences, Lafayette, CO, USA.

PMID: 40580293
DOI: 10.1007/978-1-0716-4642-7_13

Abstract

This chapter presents a protocol for short tandem repeat (STR) analysis, a robust, economical, and highly accurate method commonly used in forensics and agriculture for genetic profiling and identity determination. The protocol involves DNA isolation from FTA cards or leaf pieces, followed by PCR amplification of STR regions and fragment analysis. The number of repeats in these short tandem patterns is unique to individuals, making it useful for genotyping analysis. By analyzing STR profiles from reference samples, a reference STR table is generated, serving as a comparison tool for identifying repeat differences between the genotypes of test individuals. This comprehensive analysis of genetic variations provides valuable insights for differentiating between individuals based on their unique genetic profiles.

Keywords: Amplicon; Amplicon fragment analysis; DNA; FTA cards; Fragment; Genetic profiling; Genotyping; Leaf tissue; Lineage; PCR; Primers; Repeats; STR; Short tandem repeat (STR) analysis.

MeSH terms

DNA Fingerprinting* / methods
Genetic Variation*
Genotype
Genotyping Techniques* / methods
Humans
Microsatellite Repeats* / genetics
Polymerase Chain Reaction / methods