Energy-carbon flow reallocation in microalgae under high CO2: Platform for carbon utilization and wastewater valorization in steel industry

Jiaying Ma; Yuying Wang; Tongcai Liu; Xiaogang You; Huaqiang Chu; Xuefei Zhou; Yalei Zhang

doi:10.1016/j.biortech.2025.132896

Energy-carbon flow reallocation in microalgae under high CO₂: Platform for carbon utilization and wastewater valorization in steel industry

Bioresour Technol. 2025 Jun 26:132896. doi: 10.1016/j.biortech.2025.132896. Online ahead of print.

Authors

Jiaying Ma¹, Yuying Wang¹, Tongcai Liu¹, Xiaogang You¹, Huaqiang Chu², Xuefei Zhou³, Yalei Zhang³

Affiliations

¹ State Key Laboratory of Water Pollution Control and Green Resource Recycling, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
² State Key Laboratory of Water Pollution Control and Green Resource Recycling, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Tongji University, Shanghai 200092, China. Electronic address: chuhuaqiang@tongji.edu.cn.
³ State Key Laboratory of Water Pollution Control and Green Resource Recycling, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Tongji University, Shanghai 200092, China.

PMID: 40581057
DOI: 10.1016/j.biortech.2025.132896

Abstract

The steel industry faces dual challenges of extensive CO₂ emissions and intricate wastewater management. This study investigated a novel microalgal platform using Chlorella pyrenoidosa for simultaneous CO₂ utilization and valorization of cold-rolling emulsion wastewater. Optimal biomass production (1.80 ± 0.07 g/L) was achieved at 5 % CO₂, whereas 20 % CO₂ caused severe inhibition of growth and carbon fixation. Mechanistic investigation revealed that 20 % CO₂ triggered systemic energy restriction, evidenced by depleted intracellular ATP and downregulated metabolic pathways (TCA cycle, oxidative phosphorylation). Furthermore, essential energy was diverted to counteract oxidative and acidification stress, upregulating multiple DNA repair pathways. Concurrently, 20 % CO₂ triggered metabolic overflow that actively rerouted 16.8 % of carbon towards extracellular organic matter. A regulatory nexus was established: energy restriction drove overall inhibition, while energy reallocation induced carbon flow alteration. These insights provide a theoretical basis for engineering microalgae with enhanced tolerance for industrial carbon utilization and bioresource recovery.

Keywords: Bioresource recovery; Chlorella pyrenoidosa; Energy metabolism; Extracellular organic matter; High CO(2) stress; Metabolic overflow.