Unravelling Immunogenic Cell Death Mechanisms by Functional Proteomics Arrays

Abstract

Immunogenic Cell Death (ICD) is a type of cell death regulated by an activation of endoplasmic reticulum stress. This signal results in an activation of the adaptative immune response against the affected cell. This characteristic has been used for the design of novel oncological drugs to complement antitumor therapy with this immunogenic response. However, ICD is complex and still needs a deep and massive study to unravel the key to its mechanism. Protein microarrays are a useful tool for large-scale protein detection and analysis, and thus may be the suitable platform for this study. In this study, antibody arrays have been designed and constructed as a method to detect different proteins involved in programmed cell death pathways in order to evaluate differential intracellular signalling profiles and correlate them with ICD.

Keywords: Antibodies; Antibody microarrays; Cancer; Cell death; Functional proteomics.