Isolation and characterization of xuetonlignans L-P, previously undescribed spirobenzofuranoid dibenzocyclooctadiene lignans from the fruits of Kadsura heteroclita

Wei Su; Hanwen Yuan; Muhammad Aamer; Xudong Zhou; Kang Zhou; Ling Li; Yupei Yang; Qingling Xie; Shiqi Liu; Gang Fu; Yu Mao; Bin Li; Wei Wang

doi:10.1016/j.fitote.2025.106726

Isolation and characterization of xuetonlignans L-P, previously undescribed spirobenzofuranoid dibenzocyclooctadiene lignans from the fruits of Kadsura heteroclita

Fitoterapia. 2025 Jun 30:106726. doi: 10.1016/j.fitote.2025.106726. Online ahead of print.

Authors

Wei Su¹, Hanwen Yuan¹, Muhammad Aamer², Xudong Zhou¹, Kang Zhou¹, Ling Li³, Yupei Yang¹, Qingling Xie¹, Shiqi Liu¹, Gang Fu¹, Yu Mao¹, Bin Li⁴, Wei Wang⁵

Affiliations

¹ TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, PR China.
² TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, PR China; H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
³ College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha 410208, PR China.
⁴ TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, PR China. Electronic address: libin@hnucm.edu.cn.
⁵ TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, PR China. Electronic address: wangwei402@hotmail.com.

PMID: 40602632
DOI: 10.1016/j.fitote.2025.106726

Abstract

Five previously undescribed spirobenzofuranoid dibenzocyclooctadiene lignans, xuetonlignans L-P (1-5), and three known dibenzocyclooctadiene lignans (6-8) were isolated from the fruits of Kadsura heteroclita. Their structures were elucidated by analysis of HR-ESI-MS, NMR spectroscopy, CD spectra and single-crystal X-ray structure determination. The hepatoprotective activity against H₂O₂-induced toxicity in HepG2 cells and anti-inflammatory activity against NO (nitric oxide) were performed. Compounds 4 and 8 showed potent hepatoprotective activity against H₂O₂-induced toxicity in HepG2 cells compared to the positive control, bicyclol. Furthermore, compound 6 significantly inhibited the NO production in LPS-induced RAW264.7 cells at a concentration of 20 μM.

Keywords: Anti-inflammatory activity; Hepatoprotective activity; Kadsura heteroclita; Spirobenzofuranoid dibenzocyclooctadiene lignans.