METTL3 Exacerbates Intimal Hyperplasia by Facilitating m6A-YTHDC1-Dependent SGK1 Gene Transcription

Jiaqi Huang; Qianqian Feng; Zhigang Dong; Zhuofan Li; Yihan Liu; Ran Xu; Zhujiang Liu; Qianhui Ding; Xueyuan Yang; Fang Yu; Yiting Jia; Yuan Zhou; Wei Kong; Hao Tang; Yi Fu

doi:10.1161/ATVBAHA.125.322961

METTL3 Exacerbates Intimal Hyperplasia by Facilitating m⁶A-YTHDC1-Dependent SGK1 Gene Transcription

Arterioscler Thromb Vasc Biol. 2025 Jul 3. doi: 10.1161/ATVBAHA.125.322961. Online ahead of print.

Authors

Jiaqi Huang^#^{1

2}, Qianqian Feng^#^{1

2}, Zhigang Dong^{1

2}, Zhuofan Li^{1

2}, Yihan Liu^{1

2}, Ran Xu^{3

4}, Zhujiang Liu^{1

2}, Qianhui Ding^{1

2}, Xueyuan Yang^{1

2}, Fang Yu^{1

2}, Yiting Jia^{1

2}, Yuan Zhou^{1

2}, Wei Kong^{1

2}, Hao Tang⁵, Yi Fu^{1

2}

Affiliations

¹ Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China. (J.H., Q.F., Z.D., Z. Li, Y.L., Z. Liu, Q.D., X.Y., F.Y., Y.J., Y.Z., W.K., Y.F.).
² State Key Laboratory of Vascular Homeostasis and Remodeling, Peking University, Beijing, China. (J.H., Q.F., Z.D., Z. Li, Y.L., Z. Liu, Q.D., X.Y., F.Y., Y.J., Y.Z., W.K., Y.F.).
³ Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing, China (R.X.).
⁴ China International Neuroscience Institute (China-INI), Beijing (R.X.).
⁵ Zhengzhou Key Laboratory of Cardiovascular Aging, National Health Commission Key Laboratory of Cardiovascular Regenerative Medicine, Central China Fuwai Hospital of Zhengzhou University, Fuwai Central China Cardiovascular Hospital and Central China Branch of the National Center for Cardiovascular Diseases, Henan (H.T.).

^# Contributed equally.

PMID: 40605746
DOI: 10.1161/ATVBAHA.125.322961

Abstract

Background: Vascular smooth muscle cell (VSMC) migration and proliferation substantially contribute to neointimal hyperplasia related to in-stent restenosis. N⁶-methyladenosine (m⁶A) modification catalyzed by the METTL3 (methyltransferase-like 3)-containing methyltransferase complex is the most abundant RNA epigenetic modification in eukaryotes, but the role of m⁶A RNA methylation in VSMC migration and proliferation and neointima formation remains highly controversial.

Methods: Primary human and rat VSMCs were utilized for in vitro experiments. VSMC-specific METTL3 knockout mice (Mettl3^flox/floxMyh11-CreER^T2) were generated to explore the role of METTL3 in carotid artery wire injury in vivo. Methylated RNA immunoprecipitation sequencing was performed to screen for genes targeted for METTL3-catalyzed m⁶A RNA methylation. Methylation site mapping, methylated RNA immunoprecipitation-quantitative polymerase chain reaction, chromatin immunoprecipitation-quantitative polymerase chain reaction, and reporter gene assays were used to explore how METTL3 modulates target gene expression.

Results: METTL3 expression was consistently upregulated in the neointima of mice subjected to carotid wire injury and in those of patients who underwent carotid endarterectomy. VSMC-specific METTL3 deficiency significantly attenuated neointima formation in mouse carotid arteries after wire injury. Accordingly, METTL3 ablation markedly repressed VSMC proliferation both in vitro and in vivo. Mechanistically, METTL3 directly catalyzed the m⁶A methylation of SGK1 (serum/glucocorticoid-regulated kinase 1) mRNA and subsequently facilitated its transcription, a process that was dependent on the established association between the SGK1 transcript and SGK1 promoter DNA via recruitment of the m⁶A reader YTHDC1 (YT521-B homology domain-containing protein 1). Conversely, SGK1 overexpression abolished the METTL3 deficiency-mediated suppression of VSMC proliferation and postinjury neointima formation.

Conclusions: METTL3-catalyzed m⁶A RNA methylation promoted VSMC proliferation and exacerbated postinjury neointima formation by facilitating YTHDC1-dependent SGK1 gene transcription. Targeting the METTL3-YTHDC1-SGK1 axis to modulate VSMC proliferation may be a potential strategy for in-stent restenosis therapy.

Keywords: N6-methyladenosine (m6A); cell proliferation; hyperplasia; methyltransferases; neointima.