Targeting liposomes and an accelerated CRISPR system for selective imaging of miR-21 in cells

Wenyue Zhang; Mengting Wu; Qingqing Zhang; Xibin Chen; Yingshu Guo

doi:10.1039/d5ay00619h

Targeting liposomes and an accelerated CRISPR system for selective imaging of miR-21 in cells

Anal Methods. 2025 Jul 3. doi: 10.1039/d5ay00619h. Online ahead of print.

Authors

Wenyue Zhang¹, Mengting Wu¹, Qingqing Zhang¹, Xibin Chen², Yingshu Guo¹

Affiliations

¹ School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China. yingshug@126.com.
² Shandong GuoChen Biotechnology Co., Ltd, Jinan 250353, China.

PMID: 40607530
DOI: 10.1039/d5ay00619h

Abstract

This study demonstrates a liposome-targeted and accelerated CRISPR strategy for specific response of miR-21 within MCF-7 cells. Targeting was achieved with the help of modification of the MUC1 aptamer. MnO₂ NS could be used as both a delivery vehicle and an accelerator for the CRISPR system, and finally to achieve spatially selective imaging of miR-21.