MINFLUX: μs and nm precision 3D tracking of dynamic lipid mobility on nanoparticles

Nanoscale. 2025 Jul 3. doi: 10.1039/d5nr00948k. Online ahead of print.

Abstract

MINFLUX microscopy opened unique opportunities to investigate complex molecular systems at a single molecule level with μs temporal and nm spatial resolutions in 3D. Here, we demonstrate its use for the characterization of nanomaterials, providing a framework for MINFLUX imaging and data analysis tailored to understanding the complex dynamics of nanosized Silica-Supported Lipid Bilayers (SSLBs).