The total antioxidant capacity of fruit was determined by TAMzyme pseudo-peroxidase activity colorimetry

Xinli Guo; Shuangshuang Yan; Nishan Jin; Wenbin Zhong; Dongying An; Murui Ying; Yutian Zhou; Yudong Wu; Xin Li; Yang Zhang

doi:10.1016/j.talanta.2025.128517

The total antioxidant capacity of fruit was determined by TAMzyme pseudo-peroxidase activity colorimetry

Talanta. 2025 Jun 26:296:128517. doi: 10.1016/j.talanta.2025.128517. Online ahead of print.

Authors

Xinli Guo¹, Shuangshuang Yan², Nishan Jin³, Wenbin Zhong¹, Dongying An¹, Murui Ying¹, Yutian Zhou¹, Yudong Wu², Xin Li⁴, Yang Zhang⁵

Affiliations

¹ School of Basic Medicine, Shenyang Medical College, 146 Huanghe North Avenue, Shenyang, 110034, China.
² School of Public Health, Shenyang Medical College, 146 Huanghe North Avenue, Shenyang, 110034, China.
³ Shenyang Key Laboratory of Medical Molecular Theranostic Probes in School of Pharmacy, Shenyang Medical College, 146 Huanghe North Avenue, Shenyang, 110034, China.
⁴ Liaoning Province Key Laboratory for Phenomics of Human Ethnic Specificity and Critical Illness, Shenyang Medical College, 146 Huanghe North Avenue, Shenyang, 110034, China; School of Stomatology, Shenyang Medical College, Shenyang, 110034, China; Shenyang Key Laboratory of Prevention and Treatment of Systemic Important Diseases Associated with Oral Diseases, Shenyang, 110034, China. Electronic address: httplixin@163.com.
⁵ Shenyang Key Laboratory of Medical Molecular Theranostic Probes in School of Pharmacy, Shenyang Medical College, 146 Huanghe North Avenue, Shenyang, 110034, China; Liaoning Province Key Laboratory for Phenomics of Human Ethnic Specificity and Critical Illness, Shenyang Medical College, 146 Huanghe North Avenue, Shenyang, 110034, China. Electronic address: zhangyangpro@symc.edu.cn.

PMID: 40609486
DOI: 10.1016/j.talanta.2025.128517

Abstract

The total antioxidant capacity (TAC) of fruits is a key indicator for measuring their quality and nutritional value. Traditional detection methods are time-consuming and cumbersome, failing to meet the requirements for rapid detection. In this study, a novel triple metal organic framework nanozyme (TAMzyme) with superior peroxidase-like (POD-like) activity is constructed for colorimetric detection of TAC. Owing to the Fe, Co, Ni doping and unique long spindle structures, the TAMzyme possesses more surface active oxygen species, negative surface charges and rapid mass transport, thus resulting in higher catalytic activity and reaction rate. Thanks to the remarkable peroxidase-like activity, the antioxidant capacity of ascorbic acid (AA), cysteine (Cys), glutathione (Glu), and gallic acid (GA) was evaluated with a detection limit between 0.54 and 1.58 μM. Antioxidant capacity was quantified in μM Trolox Equivalent (TE), with Trolox as the standard reference compound. The sensor accurately determined the TAC in kiwi fruit (194.97 ± 2.73 μM TE) and oranges (205.10 ± 3.85 μM TE), aligning with results from a standardized ABTS kit. This study presents a rapid, cost-effective, and straightforward method for quantifying TAC in complex food matrices.

Keywords: Colorimetric sensor; Fruit; Nanozymes; Peroxidase-like activity; Total antioxidant capacity.