Iridoids and other constituents from the aerial parts of Patrinia rupestris and their inhibitory effects on nitric oxide production

Zhi-Yuan Liu; Sheng-Run Guo; Jian-Xian Tang; Chun-Yan Zhao; Li Pu; Li-Zhong Wang; Dan Liu; Rong-Tao Li; Hong-Mei Li

doi:10.1016/j.phytochem.2025.114609

Iridoids and other constituents from the aerial parts of Patrinia rupestris and their inhibitory effects on nitric oxide production

Phytochemistry. 2025 Jul 1:114609. doi: 10.1016/j.phytochem.2025.114609. Online ahead of print.

Authors

Zhi-Yuan Liu¹, Sheng-Run Guo¹, Jian-Xian Tang¹, Chun-Yan Zhao¹, Li Pu¹, Li-Zhong Wang¹, Dan Liu¹, Rong-Tao Li¹, Hong-Mei Li²

Affiliations

¹ Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan, P. R. China.
² Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan, P. R. China. Electronic address: lihongmei0823@163.com.

PMID: 40609848
DOI: 10.1016/j.phytochem.2025.114609

Abstract

Forty-three compounds, including nine undescribed compounds, five iridoids (patrirupesins A-E, 1-5) and four bis-iridoids (patrirupesins F-I, 6-9), were isolated from the EtOAc-soluble fraction of the aerial parts of Patrinia rupestris (Pall.) Dufr., and their structures were elucidated based on Mass spectrometry and NMR spectroscopy. The absolute configuration of patrirupesins A-E (1-5) and I (9) was determined by experimental and calculated electronic circular dichroism (ECD). In addition, patrirupesin E (5), patrirupesin H (8), 8,9-didehydro-7-hydroxydolichodial (17), 5,6-epoxy-3-hydroxy-7-megastigmen-9-one (19), iso-seco-tanapartholide (24) and 3-hydroxy-2-methyl-4-(3-oxoprop-1-en-2-yl)cyclopent-1-enecarbaldehyde (43) showed in vitro anti-inflammatory activity on NO production in RAW 264.7 cells stimulated by lipopolysaccharide (LPS) with IC₅₀ values of 9.32 ± 3.83, 4.63 ± 1.23, 8.31 ± 4.05, 17.23 ± 5.19, 8.30 ± 6.17 and 27.38 ± 4.69 μM, respectively.

Keywords: Bis-iridoids; Caprifoliaceae; Inhibition on NO production; Iridoids; Patrinia rupestris.