Objective: To study the genetic polymorphism of red blood cell blood group among in Shenzhen Han, Indian and Xinjiang Uyghur populations, to provide scientific basis for the demand prediction and collection strategy of rare blood group, and to explore the genetic differences of blood group between Han and Caucasians.
Methods: The haplotypes of antigen coding genes of 10 target blood group systems from 87 Han Chinese and 50 Indian blood donors in Shenzhen, and 49 healthy Uyghur people in Xinjiang were obtained by three-generation sequencing technology, and the polymorphism and frequency characteristics were analyzed.
Results: Only a single genotype was detected the Langereis and Vel blood group systems in samples from three different populations. Only one genotype of Dombrock blood group was detected in Shenzhen Han, and Junior blood group in Xinjiang Uygur populations. In the MNS, Duffy, Kidd, Dombrock and Junior blood group systems, the haplotype frequency of Indian and Uyghur people was significantly different from that of Han people. Compared with the Han ethnic group, the rare blood group s-, Fy(a-), Jk(a-b-), and Do(a+b-) have a higher frequency among the Uyghur and Indian populations.
Conclusion: Haplotype frequencies of antigen genes for MNS, Duffy, Kidd, Dombrock and Junior blood group system in Shenzhen Han, Indian and Uyghur populations displayed a polymorphic difference with unique distribution characteristics different from the ethnic groups in other regions.
题目: 深圳地区汉族、印度人与新疆维吾尔族人群红细胞血型基因单倍型频率差异研究.
目的: 研究深圳地区的汉族、印度人及新疆维吾尔族人群的红细胞血型基因多态性，为稀有血型血液的需求预测和采集策略提供科学依据，并探索汉族与高加索人种间的血型遗传差异。.
方法: 选取深圳87例汉族和50例印度献血者血液样本，以及49例新疆维吾尔族健康体检者血液样本，采用三代测序技术，获取10个目标血型系统的抗原编码基因单倍型，分析多态性和频率特征。.
结果: Langereis和Vel血型系统在3个不同群体的样本中仅检测到单一基因型；Dombrock血型在深圳汉族、以及Junior血型在新疆维吾尔族样本中亦只有一种基因型。在MNS、Duffy、Kidd、Dombrock和Junior血型系统中，印度人群和维吾尔族人群的单倍型频率与深圳汉族人群具有显著差异。与深圳汉族人群相比，新疆维吾尔族和印度人群中s-、Fy(a-)、Jk(a-b-)和Do(a+b-)稀有血型的频率较高。.
结论: 在深圳汉族与印度人及维吾尔族人群中MNS、Duffy、Kidd、Dombrock和Junior血型系统抗原基因单倍型频率具有多态性差异，与其他地区不同民族相比具有独特的分布特点。.
Keywords: blood group gene; haplotype frequency; rare blood group; population difference; Han.