Androgen receptor expression in triple negative breast cancer: A report from a tertiary care center

J Cancer Res Ther. 2025 Apr 1;21(3):551-557. doi: 10.4103/jcrt.jcrt_461_24. Epub 2025 Jul 5.

Abstract

Context: The role of androgen receptor (AR) as a prognostic marker in triple negative breast cancer (TNBC) has been ambiguous since existing reports illustrate conflicting results.

Aims: To compare clinicopathological features and survival between AR-positive TNBC and QNBC.

Methods and material: A total of 281 subjects were included and tissue microarrays (TMA) were constructed using tumor tissue cores from their formalin-fixed paraffin-embedded blocks. The testing of AR expression was done using immunohistochemistry on TMA slides. Tumors with at least 1% nuclear staining were considered to be positive for AR expression. Comprehensive data were gathered from electronic medical records and analyzed using statistical tests.

Results: The expression of AR was positive in 52 cases. The TNBC subgroup with a positive family history of cancer had a significantly higher frequency (P = 0.011) of second primary occurrence. AR expression was significantly associated with higher age at diagnosis (P = 0.006), smaller tumor size (P = 0.033), lower tumor grade (P < 0.001), non-basal-like phenotype (P < 0.001), and distant recurrence (P = 0.024). At a median follow-up period of 88 (range 0-137) months, a 10-year disease-free rate was higher in quadruple negative breast cancer (QNBC) (78% vs. 73%) compared to AR-positive TNBC. Likewise, the 10-year overall survival rate in QNBC was superior (75% vs. 72%) to AR-positive TNBC.

Conclusions: AR is expressed in one-fifth of all TNBC. AR expression is associated with smaller tumor size, lower tumor grade, higher age, and distant recurrence. AR-positive TNBC exhibits shorter disease-free survival and overall survival than QNBC.

Keywords: Androgen expression; disease-free survival; overall survival; quadruple negative breast cancer; triple-negative breast cancer.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor* / metabolism
  • Female
  • Follow-Up Studies
  • Humans
  • Immunohistochemistry
  • Middle Aged
  • Neoplasm Grading
  • Neoplasm Recurrence, Local / pathology
  • Neoplasm Staging
  • Prognosis
  • Receptors, Androgen* / genetics
  • Receptors, Androgen* / metabolism
  • Tertiary Care Centers
  • Tissue Array Analysis
  • Triple Negative Breast Neoplasms* / genetics
  • Triple Negative Breast Neoplasms* / metabolism
  • Triple Negative Breast Neoplasms* / mortality
  • Triple Negative Breast Neoplasms* / pathology

Substances

  • Receptors, Androgen
  • AR protein, human
  • Biomarkers, Tumor