Chemical and microbial mechanisms underpinning calcium-regulated suppression of cadmium bioavailability in alkaline paddy soil and cadmium accumulation in rice grain

Xiaoyu Feng; Zhihao Pang; Junli Liu; Hongyun Peng; Yongchao Liang; Bin Guo

doi:10.1016/j.ecoenv.2025.118620

Chemical and microbial mechanisms underpinning calcium-regulated suppression of cadmium bioavailability in alkaline paddy soil and cadmium accumulation in rice grain

Ecotoxicol Environ Saf. 2025 Jul 4:302:118620. doi: 10.1016/j.ecoenv.2025.118620. Online ahead of print.

Authors

Xiaoyu Feng¹, Zhihao Pang², Junli Liu³, Hongyun Peng², Yongchao Liang⁴, Bin Guo⁵

Affiliations

¹ Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, State Key Laboratory for Quality and Safety of Agro-Products, College of Environmental Resource Sciences, Zhejiang University, Hangzhou 310058, China; Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
² Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, State Key Laboratory for Quality and Safety of Agro-Products, College of Environmental Resource Sciences, Zhejiang University, Hangzhou 310058, China.
³ Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
⁴ Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, State Key Laboratory for Quality and Safety of Agro-Products, College of Environmental Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: ycliang@zju.edu.cn.
⁵ Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address: guob@zaas.ac.cn.

PMID: 40617224
DOI: 10.1016/j.ecoenv.2025.118620

Abstract

Lime is an effective amendment for reducing cadmium (Cd) bioavailability in acidic soils. However, the mechanisms underlying calcium (Ca)-Cd interactions during liming remain unclear. Here, the effects of different lime materials (CaO, Ca(OH)₂, and CaCO₃) at three amendment levels (0.05 %, 0.10 % and 0.25 %) on soil bacterial communities, Cd and Ca uptake, and rice growth and yield were investigated in alkaline paddy soils. The dynamics of pH, Ca and Cd concentrations in soil pore water were also determined. Results indicated that all liming treatments significantly enhanced rice growth and yield by 6.71 %-44.83 %, but reduced Cd uptake and translocation by 29.61 %-60.16 %, compared with the control. The most pronounced effect was observed in the treatment with 0.05 % CaCO₃, which decreased grain Cd by 56.82 %. This treatment also maintained the highest soil pore water pH and the lowest Cd concentration throughout the experimental period. In addition, CaCO₃ application significantly decreased the richness and diversity of soil bacterial communities. Specifically, Desulfobacterota, Gemmatimonadota, and Bacteroidota nearly disappeared, while the relative abundance of WPS-2 and Chloroflexi increased. Overall, CaCO₃ is more effective than CaO and Ca(OH)₂ in controlling Cd bioavailability in alkaline soils, and an application rate of 0.05 % is sufficient. The associated mechanisms are due to the antagonism of Ca with Cd in the soil-rice system, and the sufficient supply of Ca or carbon (C) resources provided by CaCO₃ for Cd resistant bacteria, thus decreasing the biological effectiveness of Cd in soils.

Keywords: Alkaline soil; Cadmium; Calcium; Lime; Rice.