Discrimination of Smoke-Exposed Pinot Noir Wines by Volatile Phenols and Volatile Phenol-Glycosides

Molecules. 2025 Jun 24;30(13):2719. doi: 10.3390/molecules30132719.

Abstract

This study investigated the correlation between five primary volatile phenols (VPs) and their glycosides in smoke-exposed and non-smoke-exposed Pinot noir wines to assess and identify potential markers for smoke taint. The results showed that all putative VP-glycosides in smoke-exposed wines were higher than in non-smoke-exposed wines, with a fold change ranging from 2.11 to 31.88 for the top fifteen differentiations. VP-glycosides showed strong positive correlations among themselves, with correlation coefficients of 0.94 for hexose-guaiacol vs. pentose (P)-hexose (H)-cresol and 0.92 for syringyl-β-D-glucopyranoside vs. H-P-4-methylguaiacol. VP-glycosides also showed relatively high correlations with free and strong acid-hydrolyzed VPs. The correlation coefficient between H-P-guaiacol and free-form guaiacol is 0.71, and between H-P-guaiacol and total guaiacol is 0.78. The strong correlation suggests that these compounds are interconnected and regulated by the severity of smoke exposure. Multivariate analysis effectively differentiated smoke-exposed wines from non-smoke-exposed ones. However, more research is needed to fill the gaps in understanding smoke-derived compounds.

Keywords: Pinot noir; high-performance liquid chromatography combined with high-resolution accurate Tandem mass spectrometry (HPLC–HR-MS/MS); smoke taint; smoke-exposure; solid-phase microextraction and gas chromatography-mass spectrometry (SPME-GC-MS); volatile phenol; volatile phenol-glycoside.

MeSH terms

  • Glycosides* / analysis
  • Glycosides* / chemistry
  • Phenols* / analysis
  • Phenols* / chemistry
  • Smoke* / analysis
  • Vitis* / chemistry
  • Volatile Organic Compounds* / analysis
  • Volatile Organic Compounds* / chemistry
  • Wine* / analysis

Substances

  • Glycosides
  • Phenols
  • Smoke
  • Volatile Organic Compounds