Development and characterization of a mouse-panda chimeric antibody with neutralizing activity against canine distemper virus

Zhengguo Wang; Guishan Ye; Kuijing He; Cong Cai; Anding Zhang; Long Li; Li Han

doi:10.1016/j.jviromet.2025.115222

Development and characterization of a mouse-panda chimeric antibody with neutralizing activity against canine distemper virus

J Virol Methods. 2025 Jul 11:115222. doi: 10.1016/j.jviromet.2025.115222. Online ahead of print.

Authors

Zhengguo Wang¹, Guishan Ye¹, Kuijing He¹, Cong Cai¹, Anding Zhang², Long Li³, Li Han⁴

Affiliations

¹ National Key Laboratory of Agricultural Microbiology，Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China.
² National Key Laboratory of Agricultural Microbiology，Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture of the People's Republic of China, Wuhan, Hubei 430070, China; International Research Center for Animal Disease, Ministry of Science and Technology of the People's Republic of China, Wuhan, Hubei 430070, China.
³ National Key Laboratory of Agricultural Microbiology，Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture of the People's Republic of China, Wuhan, Hubei 430070, China; International Research Center for Animal Disease, Ministry of Science and Technology of the People's Republic of China, Wuhan, Hubei 430070, China. Electronic address: lilong@mail.hzau.edu.cn.
⁴ National Key Laboratory of Agricultural Microbiology，Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture of the People's Republic of China, Wuhan, Hubei 430070, China; International Research Center for Animal Disease, Ministry of Science and Technology of the People's Republic of China, Wuhan, Hubei 430070, China. Electronic address: hanli209@mail.hzau.edu.cn.

PMID: 40653203
DOI: 10.1016/j.jviromet.2025.115222

Abstract

Canine distemper (CD) poses as the primary virulent infectious disease threatening the safety of panda populations, and monoclonal antibodies (mAbs) represent a potential therapeutic option for the treatment of CD in pandas. To avoid immune rejection of mouse-derived mAbs in panda clinical treatment, this study utilized a mouse-derived mAb targeting the CDV-H protein to develop a mouse-panda chimeric antibody and established a CHO cell line with stable high-expression of this chimeric antibody. SDS-PAGE results indicated that the chimeric antibody expressed by CHO cells was correctly expressed. Both indirect ELISA and indirect immunofluorescence experiments demonstrated that the chimeric antibody specifically recognized the CDV-H protein and CDV virus. Furthermore, neutralization experiments confirmed its neutralizing effect on CDV. This study successfully prepared the panda-derived chimeric antibody, providing a potential candidate drug reserve for the treatment of CD in pandas.

Keywords: CHO expression system; canine distemper; mouse-panda chimeric antibody.