Natural diosmin alleviating obesity and nonalcoholic fatty liver disease by regulating the activating the AMP-activated protein kinase (AMPK) pathway

Chin J Nat Med. 2025 Jul;23(7):863-870. doi: 10.1016/S1875-5364(25)60914-9.

Abstract

Obesity and metabolic dysfunction-associated steatotic liver disease (MASLD) are linked to numerous chronic conditions, including cardiovascular disease, atherosclerosis, chronic kidney disease, and type II diabetes. Previous research identified the natural flavonoid diosmin, derived from Chrysanthemum morifolium, as a regulator of glucose metabolism. However, its effects on lipid metabolism and underlying mechanisms remained unexplored. The AMP-activated protein kinase (AMPK) pathway serves a critical function in glucose and lipid metabolism. The relationship between diosmin and the AMPK pathway has not been previously documented. This investigation examined diosmin's capacity to reduce lipid content through AMPK pathway activation in hepatoblastoma cell line G2 (HepG2) and 3T3-L1 cells. The study revealed that diosmin inhibits lipogenesis, indicating its potential as an anti-obesity agent in obese mice. Moreover, diosmin demonstrated effective MASLD alleviation in vivo. These findings suggest that diosmin may represent a promising therapeutic candidate for treating obesity and MASLD.

Keywords: AMP-activated protein kinase; Diosmin; Lipometabolism; Metabolic dysfunction-associated steatotic liver disease; Obesity.

MeSH terms

  • 3T3-L1 Cells
  • AMP-Activated Protein Kinases* / genetics
  • AMP-Activated Protein Kinases* / metabolism
  • Animals
  • Chrysanthemum / chemistry
  • Diosmin* / administration & dosage
  • Hep G2 Cells
  • Humans
  • Lipid Metabolism / drug effects
  • Lipogenesis / drug effects
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Non-alcoholic Fatty Liver Disease* / drug therapy
  • Non-alcoholic Fatty Liver Disease* / enzymology
  • Non-alcoholic Fatty Liver Disease* / metabolism
  • Obesity* / drug therapy
  • Obesity* / enzymology
  • Obesity* / metabolism
  • Signal Transduction / drug effects

Substances

  • Diosmin
  • AMP-Activated Protein Kinases