Proteins of vesicular stomatitis virus. I. Polyacrylamide gel analysis of viral antigens

J Virol. 1969 Apr;3(4):404-13. doi: 10.1128/JVI.3.4.404-413.1969.

Abstract

Infection of L cells with vesicular stomatitis virus results in the release, into the cell-free fluid, of four antigenic components separable by rate zonal centrifugation on sucrose gradients. The largest antigens are the infectious (B) particle and a shorter noninfectious, autointerfering (T) particle. The two small antigens are characterized by sedimentation coefficients of approximately 20S and 6S. Treatment of purified B or T particles with sodium deoxycholate results in the release from the particle of a nucleoprotein core which can be purified on sucrose gradient and which has a sedimentation coefficient characteristic of the virus from which it arose. Utilizing purified antigens labeled with (14)C-amino acids during growth, we examined the protein constituents of each antigen by acrylamide-gel electrophoresis. The proteins of B and T particles are identical, each containing one minor (virus protein 1) and three major (virus proteins 2, 3, and 4) proteins, numbered in order of increasing mobility. Virus protein 3 originates from the nucleoprotein core, whereas proteins 2 and 4 come from the coat. The origin of virus protein 1 is not known. The 20S antigen contains a single protein equivalent to virus protein 3, whereas the 6S antigen shows a single protein which is similar to, but probably distinct from, virus protein 2.

MeSH terms

  • Amino Acids / metabolism
  • Antigens / analysis*
  • Antigens / isolation & purification
  • Carbon Isotopes
  • Centrifugation, Density Gradient
  • Complement Fixation Tests
  • Cycloheximide / pharmacology
  • Cytopathogenic Effect, Viral
  • Electrophoresis, Disc
  • L Cells
  • Vesicular stomatitis Indiana virus / analysis*
  • Vesicular stomatitis Indiana virus / drug effects
  • Viral Proteins / analysis*
  • Viral Proteins / isolation & purification

Substances

  • Amino Acids
  • Antigens
  • Carbon Isotopes
  • Viral Proteins
  • Cycloheximide