Analysis of the cell cycle of late erythroid progenitor cells by sedimentation at unit gravity

Stem Cells (1981). 1981;1(1):5-14.

Abstract

Approximately 70% of the late erythroid progenitor cells (E-CFU) which are present in normal bone marrow are killed by exposure to high specific activity tritiated thymidine (3H-TdR) in vitro or to hydroxyurea in vivo, indicating that a high proportion of these cells synthesize DNA. Their cell cycle was further analyzed by sedimentation at unit gravity. The modal sedimentation rate of 7.4 mm/h appeared to correspond to the fraction that is killed by 3H-TdR. Cells sedimenting at 6.0 and 9.5 mm/h were not susceptible to kill by 3H-TdR and correspond to cells in, respectively, G1 and G2/M. Based on a buoyant density of 1.077 g/cm3, the modal diameter of cells in G1 was calculated to be 8.3 micron, in S 9.2 micron and in G2/M 10.5 micron. The E-CFU population appeared to be composed of about 18% G1 cells, about 70% S phase cells and about 12% G2/M cells. The surviving fraction of E-CFU 2 h after intraperitoneal administration of 1 g/kg hydroxyurea was identified as being mainly a synchronous population in early S phase. The data are best explained by a short duration of G1 and G2/M phases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells
  • Cell Cycle
  • Cell Separation
  • Cells, Cultured
  • Centrifugation, Density Gradient / methods
  • Female
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / drug effects
  • Hydroxyurea / pharmacology
  • Interphase
  • Mice
  • Mice, Inbred Strains
  • Tritium

Substances

  • Tritium
  • Hydroxyurea