CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression

Proc Natl Acad Sci U S A. 1995 May 9;92(10):4342-6. doi: 10.1073/pnas.92.10.4342.

Abstract

Cultured human umbilical vein endothelial cells (EC) constitutively express a low level of CD40 antigen as detected by monoclonal antibody binding and fluorescence flow cytometric quantitation. The level of expression on EC is increased about 3-fold following 24 h treatment with optimal concentrations of tumor necrosis factor, interleukin 1, interferon beta, or interferon gamma; both interferons show greater than additive induction of CD40 when combined with tumor necrosis factor or interleukin 1. Expression of CD40 increases within 8 h of cytokine treatment and continues to increase through 72 h. A trimeric form of recombinant murine CD40 ligand acts on human EC to increase expression of leukocyte adhesion molecules, including E-selectin, vascular cell adhesion molecule 1, and intercellular adhesion molecule 1. CD40 may be detected immunocytochemically on human microvascular EC in normal skin. We conclude that endothelial CD40 may play a role as a signaling receptor in the development of T-cell-mediated inflammatory reactions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, CD / biosynthesis*
  • Antigens, Differentiation, B-Lymphocyte / biosynthesis*
  • CD40 Antigens
  • Cell Adhesion Molecules / biosynthesis*
  • Cells, Cultured
  • Cytokines / pharmacology*
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / immunology*
  • Gene Expression / drug effects*
  • Gene Expression / immunology
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Humans
  • Interferon-beta / pharmacology
  • Interferon-gamma / pharmacology
  • Interleukins / pharmacology
  • Kinetics
  • Mice
  • Recombinant Proteins / pharmacology
  • Skin / cytology
  • Skin / immunology*
  • Tumor Necrosis Factor-alpha / pharmacology
  • Umbilical Veins

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • CD40 Antigens
  • Cell Adhesion Molecules
  • Cytokines
  • Interleukins
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Interferon-beta
  • Interferon-gamma
  • Granulocyte-Macrophage Colony-Stimulating Factor