An enzyme-linked immunosorbent assay (ELISA) for monitoring rodent colonies for Pasteurella pneumotropica antibodies

R Boot; H C Thuis; J L Veenema; R G Bakker

doi:10.1258/002367795781088306

An enzyme-linked immunosorbent assay (ELISA) for monitoring rodent colonies for Pasteurella pneumotropica antibodies

Lab Anim. 1995 Jul;29(3):307-13. doi: 10.1258/002367795781088306.

Authors

R Boot¹, H C Thuis, J L Veenema, R G Bakker

Affiliation

¹ Section of Laboratory Animal Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.

PMID: 7564216
DOI: 10.1258/002367795781088306

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Pasteurella pneumotropica antibodies in the sera of rats, mice, hamsters and Mastomys. P. pneumotropica from mice and rats showed cross-reactivity. The ELISA using P. pneumotropica NCTC 8284 detected more infected animals than selective culture in groups of rodents from which P. pneumotropica, Haemophilus sp and/or Actinobacillus sp were cultured. Cross reactivity between P. pneumotropica NCTC 8284 and haemophilus and actinobacillus isolates were not studied.

MeSH terms

Animals
Antibodies, Bacterial / blood*
Antigens, Bacterial / immunology
Cricetinae
Cross Reactions
Enzyme-Linked Immunosorbent Assay / veterinary*
Female
Immune Sera / immunology
Mice
Pasteurella / immunology*
Pasteurella / isolation & purification
Pasteurella Infections / diagnosis
Pasteurella Infections / veterinary*
Pasteurellaceae / isolation & purification
Pasteurellaceae Infections / diagnosis
Pasteurellaceae Infections / veterinary*
Rats
Rodent Diseases / diagnosis*
Species Specificity

Substances

Antibodies, Bacterial
Antigens, Bacterial
Immune Sera